Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,724,058 |
C→T |
D444D (GAC→GAT) |
pssA → |
phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,724,058 | 0 | C | T | 97.2%
| 115.8
/ NA
| 36 | D444D (GAC→GAT) | pssA | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/17); total (19/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAG > NC_000913/2723923‑2724192
|
acaGCGAGAAAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAtcgatcga < 1:794697/139‑1 (MQ=255)
caGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATcgatcgat > 2:837730/1‑139 (MQ=255)
gATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca > 1:1092356/1‑139 (MQ=255)
aTCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAGGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATGGATTAATTAGCCGCATCCTGTAATcac > 2:713822/1‑139 (MQ=255)
cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc < 1:619983/139‑1 (MQ=255)
aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg > 2:748667/1‑139 (MQ=255)
aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc > 1:518047/1‑124 (MQ=255)
aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc < 2:518047/124‑1 (MQ=255)
cACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca < 2:942883/111‑1 (MQ=255)
cACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca > 1:942883/1‑111 (MQ=255)
tATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTg > 1:143797/1‑139 (MQ=255)
tCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGattaat < 2:1119236/87‑1 (MQ=255)
tCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGattaat > 1:1119236/1‑87 (MQ=255)
tGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGt > 1:775380/1‑139 (MQ=255)
aTTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa < 2:143797/139‑1 (MQ=255)
tGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTAtt > 2:360541/1‑139 (MQ=255)
ccGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCt > 1:225661/1‑139 (MQ=255)
ttATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttttt < 2:775380/139‑1 (MQ=255)
cGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGt > 1:847822/1‑139 (MQ=255)
cGTAAACTCATCCGCCGTTTGCGCCGTATACGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGCTTGTTTTTTGGAGGCCACGTGTTGCGTATTCTTTTTGTCTGTTCGCTATTGt < 1:837730/139‑1 (MQ=255)
cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg > 2:568914/1‑92 (MQ=255)
cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg < 1:568914/92‑1 (MQ=255)
cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCg < 2:951924/126‑1 (MQ=255)
cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCg > 1:951924/1‑126 (MQ=255)
cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCa < 2:198335/47‑1 (MQ=255)
cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCa > 1:198335/1‑47 (MQ=255)
cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa > 2:1081433/1‑107 (MQ=255)
cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa < 1:1081433/107‑1 (MQ=255)
cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCt > 2:630793/1‑139 (MQ=255)
aTCCGCCGTTTGCGCAGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGGGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTg > 2:1093413/1‑139 (MQ=255)
tCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAAcccc < 2:117546/63‑1 (MQ=255)
tCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAAcccc > 1:117546/1‑63 (MQ=255)
ccgccgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGTGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa < 2:844613/139‑1 (MQ=255)
ccgccgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa < 1:748667/139‑1 (MQ=255)
cgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa < 2:1092356/139‑1 (MQ=255)
cgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa < 1:360541/139‑1 (MQ=255)
gcgcCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat < 1:881609/139‑1 (MQ=255)
aTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCAGCCTGTACGGGGTTTGTTGTTTGGAGGCCACGTTTTGCGTATTCTCTATGTCTGTTCGCTATTGTTACTTTCTGGCAGCAACAATATGGCTa > 2:396492/1‑139 (MQ=255)
tcgatcgaTTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg > 2:370990/1‑139 (MQ=255)
|
ACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAG > NC_000913/2723923‑2724192
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
