Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,938,533 |
C→T |
R103W (CGG→TGG) |
rbsR → |
transcriptional repressor of ribose metabolism |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,938,533 | 0 | C | T | 100.0%
| 101.8
/ NA
| 30 | R103W (CGG→TGG) | rbsR | transcriptional repressor of ribose metabolism |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/14); total (16/14) |
ATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTG > NC_000913/3938405‑3938653
|
aTACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCg < 1:193605/139‑1 (MQ=255)
cATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAAt < 2:300756/139‑1 (MQ=255)
ggCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTgg > 2:235832/1‑139 (MQ=255)
ttGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGc > 1:143386/1‑139 (MQ=255)
ccAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCaaaa > 1:90865/1‑139 (MQ=255)
aCCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCCAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAAcgcg > 1:525450/1‑139 (MQ=255)
ttCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCt > 2:139294/1‑139 (MQ=255)
ttCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTg > 1:351617/1‑139 (MQ=255)
cAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGtt > 2:473422/1‑139 (MQ=255)
aGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa < 1:235832/139‑1 (MQ=255)
aGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa < 1:139294/139‑1 (MQ=255)
tGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACtgtg < 2:143386/139‑1 (MQ=255)
gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCa > 2:214743/1‑139 (MQ=255)
gTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCa > 1:79427/1‑139 (MQ=255)
gTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGa < 1:473422/139‑1 (MQ=255)
tGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGaa < 1:214743/139‑1 (MQ=255)
tCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGa > 1:536194/1‑139 (MQ=255)
cgcgGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATgg > 2:120762/1‑92 (MQ=255)
cgcgGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATgg < 1:120762/92‑1 (MQ=255)
tcGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTa < 1:56557/136‑1 (MQ=255)
tcGTCCTTTGCAATACCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTa > 2:56557/1‑136 (MQ=255)
aCCGAAGGCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACtg < 2:525450/139‑1 (MQ=255)
ggCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAAc < 2:676922/49‑1 (MQ=255)
ggCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAAc > 1:676922/1‑49 (MQ=255)
ggCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGaa > 2:13062/1‑81 (MQ=255)
ggCGATGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGaa < 1:13062/81‑1 (MQ=255)
tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACg > 1:653119/1‑108 (MQ=255)
tGAACAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACg < 2:653119/108‑1 (MQ=255)
aaCAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCt > 2:510557/1‑54 (MQ=255)
aaCAGTGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCt < 1:510557/54‑1 (MQ=255)
|
ATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTG > NC_000913/3938405‑3938653
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
