Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,574,259 |
A→G |
pseudogene (341/2937 nt) |
yjiV → |
pseudogene, conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,574,259 | 0 | A | G | 100.0%
| 137.7
/ NA
| 36 | pseudogene (341/2937 nt) | yjiV | pseudogene, conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/19); total (17/19) |
TATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG > NC_000913/4574132‑4574394
|
tatgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTg < 1:332212/139‑1 (MQ=255)
tgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGc < 1:641338/139‑1 (MQ=255)
tgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa > 2:300025/1‑139 (MQ=255)
tgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa > 1:418769/1‑139 (MQ=255)
aaCCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATtct > 1:524085/1‑139 (MQ=255)
aGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCttt > 1:340622/1‑139 (MQ=255)
aGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCttt > 2:559363/1‑139 (MQ=255)
gCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTg < 1:559363/139‑1 (MQ=255)
gACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc < 1:556427/139‑1 (MQ=255)
gACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc < 1:573237/139‑1 (MQ=255)
aGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAAc < 2:305853/139‑1 (MQ=255)
tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAgg > 2:4869/1‑81 (MQ=255)
tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAgg < 1:4869/81‑1 (MQ=255)
tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa > 2:870188/1‑139 (MQ=255)
tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACg < 1:895152/121‑1 (MQ=255)
tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACg > 2:895152/1‑121 (MQ=255)
tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCg > 2:25711/1‑139 (MQ=255)
tAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGaa > 1:462635/1‑139 (MQ=255)
gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAgg < 2:885007/87‑1 (MQ=255)
gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAgg > 1:885007/1‑87 (MQ=255)
tAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTATAGAAGAAAAAGTCGAAAAAGAGAAc < 1:127349/139‑1 (MQ=255)
aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACa > 2:128045/1‑104 (MQ=255)
aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACa < 1:128045/104‑1 (MQ=255)
aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACa > 1:79929/1‑139 (MQ=255)
ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa < 2:893005/101‑1 (MQ=255)
ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa > 1:893005/1‑101 (MQ=255)
ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTc > 1:393652/1‑139 (MQ=255)
tcttctGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCt < 1:300025/139‑1 (MQ=255)
tctGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCg < 2:418769/139‑1 (MQ=255)
tGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa < 2:295489/56‑1 (MQ=255)
tGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa > 1:295489/1‑56 (MQ=255)
gggCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGga < 2:524085/139‑1 (MQ=255)
gCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGaa > 1:788289/1‑56 (MQ=255)
gCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGaa < 2:788289/56‑1 (MQ=255)
ccAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAg < 2:462635/139‑1 (MQ=255)
cAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAgg < 2:340622/139‑1 (MQ=255)
ggggAAATAAAGGTGGCGATGAATTCTCTTTGCATGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGTCGCAGGAGAGACCAGCGAGGCAgg < 1:870188/139‑1 (MQ=255)
|
TATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG > NC_000913/4574132‑4574394
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
