Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I1 R1
|
64 |
69.7 |
3300380 |
93.4% |
3082554 |
111.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,218,665 |
T→C |
T138A (ACC→GCC) |
aer ← |
fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,218,665 | 0 | T | C | 83.7%
| 122.3
/ 14.7
| 49 | T138A (ACC→GCC) | aer | fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base C (20/21); total (23/26) |
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01 |
ATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAC > NC_000913/3218541‑3218794
|
aTGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCa < 2:782060/139‑1 (MQ=255)
gccgccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACg > 1:512559/1‑139 (MQ=255)
cgccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCt < 1:541753/139‑1 (MQ=255)
ccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCttt > 1:1252904/1‑139 (MQ=255)
cAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTg < 1:1447753/139‑1 (MQ=255)
cATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTcc < 1:583499/139‑1 (MQ=255)
gggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgc > 2:1566920/1‑139 (MQ=255)
gggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgc > 1:745447/1‑139 (MQ=255)
ggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCTAGCCAGCCTTTACGCACCACCAGACCTTTATGAATACGCGTACTGGTACGACCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgcc < 2:1048011/139‑1 (MQ=255)
atcaCTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGa > 1:290464/1‑139 (MQ=255)
gcccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCg > 2:424615/1‑139 (MQ=255)
gcccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCg > 1:1173751/1‑139 (MQ=255)
cccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGt < 2:1173751/139‑1 (MQ=255)
cgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCg < 2:290464/139‑1 (MQ=255)
ccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGcc > 2:333754/1‑139 (MQ=255)
gTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAAt < 2:1255740/139‑1 (MQ=255)
gTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAAt < 1:59917/139‑1 (MQ=255)
tAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc < 2:695212/139‑1 (MQ=255)
aGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGc > 1:434734/1‑139 (MQ=255)
ccTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCt > 2:458371/1‑139 (MQ=255)
ttACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTcgc < 1:70616/139‑1 (MQ=255)
ccaccaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt < 2:758758/139‑1 (MQ=255)
accaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTAc < 1:911610/54‑1 (MQ=255)
accaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTAc > 2:911610/1‑54 (MQ=255)
ccaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCgg > 2:758309/1‑139 (MQ=255)
aGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTa < 1:1250757/139‑1 (MQ=255)
ttATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt > 1:1496524/1‑128 (MQ=255)
ttATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt < 2:1496524/128‑1 (MQ=255)
ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGaa > 2:117050/1‑91 (MQ=255)
ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGaa < 1:117050/91‑1 (MQ=255)
ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCAt > 2:261068/1‑139 (MQ=255)
ttATGAATACCCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt < 2:44125/128‑1 (MQ=255)
ttATGAATACCCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt > 1:44125/1‑128 (MQ=255)
gAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTAAGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGc < 1:320563/139‑1 (MQ=255)
aTACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc < 2:611082/139‑1 (MQ=255)
tACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCg > 1:1393092/1‑139 (MQ=255)
tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTcc < 2:912878/109‑1 (MQ=255)
tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTcc > 1:912878/1‑109 (MQ=255)
tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCg < 2:291081/139‑1 (MQ=255)
cGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa < 2:996748/139‑1 (MQ=255)
gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc > 2:237481/1‑135 (MQ=255)
gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc < 1:237481/135‑1 (MQ=255)
gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAc > 2:1368520/1‑139 (MQ=255)
aCTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATGGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa < 2:942421/134‑1 (MQ=255)
aCTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa > 1:942421/1‑134 (MQ=255)
cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc < 1:1546020/78‑1 (MQ=255)
cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc > 2:1546020/1‑78 (MQ=255)
cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt < 2:1223589/113‑1 (MQ=255)
cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt > 1:1223589/1‑113 (MQ=255)
|
ATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAC > NC_000913/3218541‑3218794
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
