Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A8 F1 I2 R1 | 97 | 53.3 | 2484976 | 94.5% | 2348302 | 111.1 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,601,769 | G→A | E94K (GAA→AAA) | lsrA → | autoinducer 2 import ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,601,769 | 0 | G | A | 100.0% | 196.1 / NA | 56 | E94K (GAA→AAA) | lsrA | autoinducer 2 import ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/29); total (27/29) | |||||||||||
CGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAG > NC_000913/1601641‑1601904 | cGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCgctgct < 1:1077710/139‑1 (MQ=255)cGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCgctgct < 1:556135/139‑1 (MQ=255) aGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCt < 2:947555/139‑1 (MQ=255) attattGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGt < 2:985497/139‑1 (MQ=255) tattGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCg > 2:80981/1‑139 (MQ=255) tGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATa > 2:659997/1‑139 (MQ=255) ccccTGCTGATAGCCGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCCCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAACAAAACAt < 1:757297/139‑1 (MQ=255) gctgATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGt > 1:599107/1‑139 (MQ=255) tAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTggg < 1:765347/139‑1 (MQ=255) aGCGTTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTGTTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGc > 2:719857/1‑139 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGaa < 1:1175835/119‑1 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCTGGGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGaa > 2:1175835/1‑119 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa < 1:845519/110‑1 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa > 2:845519/1‑110 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACa > 2:594974/1‑122 (MQ=255) tACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACa < 1:594974/122‑1 (MQ=255) tGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGc > 1:490529/1‑98 (MQ=255) tGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGc < 2:490529/98‑1 (MQ=255) gagaTTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAAc > 1:154482/1‑139 (MQ=255) tGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGctc > 1:216599/1‑139 (MQ=255) caacaaCTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATg < 1:719857/139‑1 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCg < 1:601659/69‑1 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCg < 1:639593/69‑1 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCg > 2:601659/1‑69 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCg > 2:639593/1‑69 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATaa > 2:147820/1‑94 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATaa < 1:147820/94‑1 (MQ=255) aacaacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGc < 2:599107/139‑1 (MQ=255) caacTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCTAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAg > 1:1085759/1‑139 (MQ=255) aCGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAATCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAAt > 1:700827/1‑139 (MQ=255) aCGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAAt < 1:659997/139‑1 (MQ=255) cGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGa < 1:152647/134‑1 (MQ=255) cGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGa > 2:152647/1‑134 (MQ=255) aTTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa > 1:266000/1‑77 (MQ=255) aTTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa < 2:266000/77‑1 (MQ=255) tAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACtt > 2:936855/1‑139 (MQ=255) gCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCgctgct < 2:126284/44‑1 (MQ=255) gCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGAAACCgctgct > 1:126284/1‑44 (MQ=255) atcaGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTg > 1:1192974/1‑139 (MQ=255) tcaGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATa > 2:830774/1‑58 (MQ=255) tcaGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATa < 1:830774/58‑1 (MQ=255) caGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATaaa > 2:900772/1‑59 (MQ=255) caGCT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATaaa < 1:900772/59‑1 (MQ=255) cT‑GGGTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGt < 2:1192974/139‑1 (MQ=255) gggTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGttt < 2:1108315/139‑1 (MQ=255) ggTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTg < 1:1185580/131‑1 (MQ=255) ggTATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTg > 2:1185580/1‑131 (MQ=255) tATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGa < 1:896356/138‑1 (MQ=255) tATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGa > 2:896356/1‑138 (MQ=255) tATTTATCTCGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGAt > 1:1116125/1‑139 (MQ=255) tcGTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAg < 2:216599/139‑1 (MQ=255) gTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATa < 1:1104425/39‑1 (MQ=37) gTTCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGATa > 2:1104425/1‑39 (MQ=255) tCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa > 1:1073282/1‑35 (MQ=255) tCCCCAGAAACCGCTGCTTTTCCCAAGCCTGTCGa < 2:1073282/35‑1 (MQ=37) cAGAAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAg < 1:936855/139‑1 (MQ=255) | CGACGTTAATGAAGATTATTGCCGGTATTACCCCTGCTGATAGCGGTACGCTGGAGATTGAGGGCAACAACTACGTCAGATTAACGCCAGTTCATGCTCATCAGCT‑GGGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAG > NC_000913/1601641‑1601904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |