Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,830,359 |
T→C |
N136S (AAC→AGC) |
astA ← |
arginine succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,830,359 | 0 | T | C | 100.0%
| 89.4
/ NA
| 24 | N136S (AAC→AGC) | astA | arginine succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/14); total (10/14) |
TTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCG > NC_000913/1830234‑1830478
|
tttGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTgcg < 1:884374/139‑1 (MQ=255)
aaCGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCg < 2:20308/139‑1 (MQ=255)
tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTc > 1:110908/1‑122 (MQ=255)
tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTc < 2:110908/122‑1 (MQ=255)
tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCCCTTTGCGCCAGTCCGGGTCGAGAAACAGCg > 1:964565/1‑139 (MQ=255)
aCCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc < 1:127728/139‑1 (MQ=255)
cATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc < 2:508443/131‑1 (MQ=255)
cATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc > 1:508443/1‑131 (MQ=255)
tttCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt < 1:127524/139‑1 (MQ=255)
tttCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt < 1:903388/139‑1 (MQ=255)
tCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgtgt > 2:35493/1‑139 (MQ=255)
gCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGAt < 1:35493/139‑1 (MQ=255)
cTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACt < 1:501768/139‑1 (MQ=255)
aaaCTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc > 1:298343/1‑139 (MQ=255)
gTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc > 2:664829/1‑139 (MQ=255)
tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGaa < 1:433174/82‑1 (MQ=255)
tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGaa > 2:433174/1‑82 (MQ=255)
gcgAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt < 2:1203224/139‑1 (MQ=255)
aaaGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt < 1:664829/139‑1 (MQ=255)
cgATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAg > 2:653001/1‑139 (MQ=255)
gATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAgg > 2:550500/1‑139 (MQ=255)
tttCGACAGCAAATAGCCGCTGCCCCCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCg < 2:964565/139‑1 (MQ=255)
aaaTAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct < 1:360657/62‑1 (MQ=255)
aaaTAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct > 2:360657/1‑62 (MQ=255)
|
TTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCG > NC_000913/1830234‑1830478
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
