Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,417	G→A	Q7* (CAG→TAG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,417	0	G	A	100.0%	96.9 / NA	29	Q7* (CAG→TAG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/15); total (14/15)

TAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTC  >  NC_000913/1907330‑1907549

tAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCg                                                                                   <  1:199837/139‑1 (MQ=255)

 aGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTAAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTCGATTGTTTTCTTCGCCCGc                                                                                  >  2:811328/1‑139 (MQ=255)

   cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                                >  1:1131960/1‑139 (MQ=255)

   cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                                >  2:396065/1‑139 (MQ=255)

   cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                                >  2:148738/1‑139 (MQ=255)

   cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                                >  1:306931/1‑139 (MQ=255)

   cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                                >  1:991549/1‑139 (MQ=255)

     gagaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGc                                                                              <  1:811328/139‑1 (MQ=255)

      agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCa                                                                             <  2:991549/139‑1 (MQ=255)

       gaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCAt                                                                            <  2:1131960/139‑1 (MQ=255)

           tgtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACa                                                                        <  2:306931/139‑1 (MQ=255)

                gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGAt                                                                   <  2:1134931/139‑1 (MQ=255)

                    acaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATaaaa                                                               >  2:640955/1‑139 (MQ=255)

                       tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTg                                                            >  2:290534/1‑139 (MQ=255)

                                ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTAc                                                   <  2:246/139‑1 (MQ=255)

                                            gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCAc                                       <  1:435550/139‑1 (MQ=255)

                                            gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCAc                                       <  2:530134/139‑1 (MQ=255)

                                            gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCAc                                       <  1:148738/139‑1 (MQ=255)

                                                tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAat                                   >  2:1090086/1‑139 (MQ=255)

                                                               tAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATa                                                                           <  1:1196878/84‑1 (MQ=255)

                                                               tAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATa                                                                           >  2:1196878/1‑84 (MQ=255)

                                                                aGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCagag                   <  1:290534/139‑1 (MQ=255)

                                                                      gTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGc                                                                                  >  1:437507/1‑70 (MQ=255)

                                                                      gTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGc                                                                                  <  2:437507/70‑1 (MQ=255)

                                                                      gTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCa                                                                             <  2:1126843/75‑1 (MQ=255)

                                                                      gTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCa                                                                             >  1:1126843/1‑75 (MQ=255)

                                                                           aCCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtc        >  2:783579/1‑139 (MQ=255)

                                                                                ttAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGa                                            >  1:986862/1‑98 (MQ=255)

                                                                                ttAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGa                                            <  2:986862/98‑1 (MQ=255)

                                                                                 taacctaaccTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtcaacgtc  >  2:608726/1‑139 (MQ=255)

                                                                                  aacctaacctTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCg                                                                                   <  2:60909/57‑1 (MQ=255)

                                                                                  aacctaacctTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCg                                                                                   >  1:60909/1‑57 (MQ=255)

TAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTC  >  NC_000913/1907330‑1907549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A