Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,735	G→A	L517L (CTG→CTA)	dld →	D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,735	0	G	A	100.0%	166.6 / NA	48	L517L (CTG→CTA)	dld	D‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/24); total (24/24)

TACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGT  >  NC_000913/2223602‑2223861

tACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTAca                                                                                                                           >  2:873450/1‑139 (MQ=255)

                         ggTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:901619/139‑1 (MQ=255)

                         ggTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:811060/139‑1 (MQ=255)

                                 aGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGAATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGTGCGCAGTACCCTGCCGAGCATaa                                                                                          <  2:721771/139‑1 (MQ=255)

                                       aTTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  2:93012/125‑1 (MQ=255)

                                       aTTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  1:93012/1‑125 (MQ=255)

                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:572203/139‑1 (MQ=255)

                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:873450/139‑1 (MQ=255)

                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:737056/139‑1 (MQ=255)

                                              ccATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGG‑‑CTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGta                                                                           >  1:513834/1‑139 (MQ=255)

                                                  tttATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGtata                                                                         >  1:651882/1‑139 (MQ=255)

                                                           tATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCgg                                                                >  2:121700/1‑139 (MQ=255)

                                                             tGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTAcc                                                                                                       >  1:888141/1‑98 (MQ=255)

                                                             tGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTAcc                                                                                                       <  2:888141/98‑1 (MQ=255)

                                                                   ccATCAGGATTACATAGTGAAAAAAGCCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTg                                                        >  2:643120/1‑139 (MQ=255)

                                                                    cATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGc                                                       <  2:651882/139‑1 (MQ=255)

                                                                    cATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGc                                                       >  1:899342/1‑139 (MQ=255)

                                                                       cAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGa                                                    >  1:1088344/1‑139 (MQ=255)

                                                                         ggATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAg                                                  >  1:50113/1‑139 (MQ=255)

                                                                          gATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGt                                                 >  1:650220/1‑139 (MQ=255)

                                                                               cATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                >  1:1238185/1‑103 (MQ=255)

                                                                               cATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  2:1238185/103‑1 (MQ=255)

                                                                                 tAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          <  1:1046252/139‑1 (MQ=255)

                                                                                  aGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          >  2:539902/1‑138 (MQ=255)

                                                                                  aGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          <  1:539902/138‑1 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  1:1052513/1‑80 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  2:1052513/80‑1 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  2:812537/1‑139 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:676545/1‑139 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:605901/1‑139 (MQ=255)

                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:544961/1‑139 (MQ=255)

                                                                                          aaGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  2:240313/1‑74 (MQ=255)

                                                                                          aaGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:240313/74‑1 (MQ=255)

                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGt                                                                                   >  1:1008199/1‑85 (MQ=255)

                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGt                                                                                   <  2:1008199/85‑1 (MQ=255)

                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCg                             >  2:1144893/1‑139 (MQ=255)

                                                                                               gTGGATGTGCATGCGTTAAAAGAACAGATGCTGG‑‑CTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAcc                          <  2:513834/139‑1 (MQ=255)

                                                                                                tGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAc                           >  2:1106753/1‑139 (MQ=255)

                                                                                                 ggATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAcc                          <  1:1106753/139‑1 (MQ=255)

                                                                                                       gCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGc                    <  2:899342/139‑1 (MQ=255)

                                                                                                         aTGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCAt                  <  2:676545/139‑1 (MQ=255)

                                                                                                             gTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAAt              >  2:91643/1‑139 (MQ=255)

                                                                                                               tAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATcc            <  2:544961/139‑1 (MQ=255)

                                                                                                                    gAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGa       <  1:91643/139‑1 (MQ=255)

                                                                                                                    gAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGa       <  1:643120/139‑1 (MQ=255)

                                                                                                                       cAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGgaga                                                             <  2:1196377/82‑1 (MQ=255)

                                                                                                                       cAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTCGTATAAAGCACCGgaga                                                             >  1:1196377/1‑82 (MQ=255)

                                                                                                                         gATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGt  <  1:812537/139‑1 (MQ=255)

TACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGT  >  NC_000913/2223602‑2223861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A