Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,602,017	(C)_7→10	coding (200/2019 nt)	hyfB →	hydrogenase 4, membrane subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2602010	4 (0.080)	59 (1.100) +CCC	32/200	0.4	93.6%	coding (193/2019 nt)	hyfB	hydrogenase 4, membrane subunit
*	?	NC_000913	2602011 =	4 (0.070)	59 (1.100) +CCC	32/200	0.4	93.6%	coding (194/2019 nt)	hyfB	hydrogenase 4, membrane subunit

TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2601873‑2602010

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGG  >  NC_000913/2602011‑2602139

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TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC                                                                                                                                     >  2:872759/1‑139

  GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCC                                                                                                                                   >  2:253051/1‑139

     TCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  1:709016/139‑1

          GTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCtt                                                                                                                           <  2:399658/139‑3

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      >  2:809847/1‑139

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     <  1:1105855/139‑1

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:1146120/1‑139

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  1:116976/1‑139

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     <  2:966745/139‑1

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  1:966745/1‑139

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:1105855/1‑139

                               ACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCC                                                                                                     >  2:653470/1‑139

                                CTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  2:447124/1‑112

                                CTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  1:447124/112‑1

                                      ATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAA                                                                                               <  1:809847/139‑1

                                             TTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGC                                                                                       <  2:781611/139‑1

                                                 CGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCT                                                                                   <  1:1146120/139‑1

                                                      TCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCA                                                                              >  2:455088/1‑139

                                                             GCTGGTGGGCGGCGTTATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGG                                                                       <  1:1020752/139‑1

                                                              CTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  2:764835/82‑1

                                                              CTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  1:764835/1‑82

                                                                 GTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCT                                                                   >  1:247616/1‑139

                                                                 GTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTT‑TCCCCCCCCCTTTTGAATTTTCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCT                                                                   >  2:276276/1‑139

                                                                      CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTA                                                              >  1:179415/1‑139

                                                                      CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGC                                                                                                                >  2:1080245/1‑89

                                                                      CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGC                                                                                                                <  1:1080245/89‑1

                                                                      CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTT                                                                                                                  >  2:640949/1‑87

                                                                      CGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTT                                                                                                                  <  1:640949/87‑1

                                                                                         AGCGGTATTACGCAATTACATGCTGGTGTAACTTTAGTCGGCCGGTTTG‑CCCCCCCCCTTTTGTATTTCCCGATTTAACCCTGCGAATGGATCGCCTCTCGGCATATATGGTGCTGGTTATCTCCTTGCTGGTGGGGGT                                           <  2:116976/139‑1

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                >  1:679206/1‑53

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCC                                                                                                                                <  2:679206/53‑1

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCC                                                                                                                                 <  1:1173713/52‑1

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCC                                                                                                                                 >  2:1173713/1‑52

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGG                                            >  2:434986/1‑136

                                                                                           CGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGG                                            <  1:434986/136‑1

                                                                                            GGAATTACGCAATTAAATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTG                                        <  1:455088/139‑1

                                                                                               ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTC                                     <  1:456267/139‑1

                                                                                               ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               >  2:512539/1‑97

                                                                                               ATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               <  1:512539/97‑1

                                                                                                TTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCT                                    <  2:247616/139‑1

                                                                                                         TACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCA                           <  1:749974/139‑1

                                                                                                              GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGAC                      <  1:653470/139‑1

                                                                                                              GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      >  2:364652/1‑59

                                                                                                              GCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACC                                                                                                      <  1:364652/59‑1

                                                                                                                TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTT                    >  2:275327/1‑139

                                                                                                                TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTG                                                <  2:67061/111‑1

                                                                                                                TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTG                                                >  1:67061/1‑111

                                                                                                                TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTC                                                                                  >  2:218428/1‑77

                                                                                                                TGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTC                                                                                  <  1:218428/77‑1

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTAT                  >  1:1034602/1‑139

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTAT                  >  2:1078301/1‑139

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               >  2:431144/1‑78

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGC                                                                               <  1:431144/78‑1

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCG                                                                                                 <  2:1228335/60‑1

                                                                                                                  GTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCG                                                                                                 >  1:1228335/1‑60

                                                                                                                            AGTCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAAT        >  2:981988/1‑139

                                                                                                                              TCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC      <  1:981988/139‑1

                                                                                                                              TCGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC      <  1:253051/139‑1

                                                                                                                               CGCCCGTTTTG‑CCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACG     <  1:276276/139‑1

                                                                                                                                   CGTTTTGCCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATTCGCGAATACGAGG  >  2:640841/1‑139

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TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2601873‑2602010

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGG  >  NC_000913/2602011‑2602139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A