Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,279	G→A	V170V (GTG→GTA)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,279	0	G	A	100.0%	111.2 / NA	33	V170V (GTG→GTA)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/15); total (18/15)

AGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAA  >  NC_000913/2811149‑2811410

aGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGa                                                                                                                             >  1:69824/1‑139 (MQ=255)

    tttACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCg                                                                                                                         <  1:67129/139‑1 (MQ=255)

           ccGCAGCATAACTGCCTGGATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCt                                                                                                                  >  1:814973/1‑139 (MQ=255)

             gcagcaTAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTaa                                                                                                                >  2:691724/1‑139 (MQ=255)

                         cctgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCg                                                                                                    <  1:691724/139‑1 (MQ=255)

                           tgcATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCt                                                                                                  >  1:550707/1‑139 (MQ=255)

                             cATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCa                                                                                                >  2:950724/1‑139 (MQ=255)

                                 aaCTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCaaaaa                                                                                            <  1:950724/139‑1 (MQ=255)

                                           cgcgCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTta                                                                                  >  1:928259/1‑139 (MQ=255)

                                            gcgcTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTtat                                                                                 <  1:567935/139‑1 (MQ=255)

                                             cgcTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTtata                                                                                >  1:472684/1‑139 (MQ=255)

                                                          aGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGGCCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGa                                                                   <  1:783638/139‑1 (MQ=255)

                                                            aaaaaGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACt                                                                 >  2:538464/1‑139 (MQ=255)

                                                                 gATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCa                                                            >  1:1149611/1‑139 (MQ=255)

                                                                     aGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCAt                                                        >  1:195974/1‑139 (MQ=255)

                                                                       cTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCa                                                                                                >  2:1141130/1‑97 (MQ=255)

                                                                       cTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCa                                                                                                <  1:1141130/97‑1 (MQ=255)

                                                                       cTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGc                                                              >  1:482121/1‑131 (MQ=255)

                                                                       cTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGc                                                              <  2:482121/131‑1 (MQ=255)

                                                                                aTCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATc                                                                                          >  1:1136610/1‑94 (MQ=255)

                                                                                aTCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATc                                                                                          <  2:1136610/94‑1 (MQ=255)

                                                                                          aaTTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTg                                   <  2:195974/139‑1 (MQ=255)

                                                                                                    ccGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTGGAAGCGATGATCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAATCAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGt                         >  1:786750/1‑139 (MQ=255)

                                                                                                    ccGTCTCGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGt                         <  1:534792/139‑1 (MQ=255)

                                                                                                       tctcGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCg                                                                                                    >  2:1181082/1‑61 (MQ=255)

                                                                                                       tctcGACCAGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCg                                                                                                    <  1:1181082/61‑1 (MQ=255)

                                                                                                               aGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATTACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCg              <  2:814973/139‑1 (MQ=255)

                                                                                                               aGATGGAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCg              <  1:994013/139‑1 (MQ=255)

                                                                                                                   ggAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGt                         >  1:745839/1‑124 (MQ=255)

                                                                                                                   ggAACAAGACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGt                         <  2:745839/124‑1 (MQ=255)

                                                                                                                          gACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTaag    >  1:590680/1‑138 (MQ=255)

                                                                                                                          gACGGTGTAGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTaag    <  2:590680/138‑1 (MQ=255)

                                                                                                                           aCGGTGTAGTTCTCGAAGCGATGAGCTGACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTaagaa  >  2:739455/1‑139 (MQ=255)

                                                                                                                               tgtaGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATaaaa                                                                             <  1:578990/60‑1 (MQ=255)

                                                                                                                               tgtaGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATaaaa                                                                             >  2:578990/1‑60 (MQ=255)

AGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAAGACGGTGTGGTTCTCGAAGCGATGAGCTAACGCGTCATCTCGCTCAAAAATCCAGATTTATAAAAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAA  >  NC_000913/2811149‑2811410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A