Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,899	T→C	intergenic (‑39/+160)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,899	0	T	C	100.0%	108.1 / NA	30	intergenic (‑39/+160)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/17); total (13/17)

GCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTT  >  NC_000913/2817764‑2818034

gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGccact                                                                                                                                      <  1:190043/139‑1 (MQ=255)

    aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAg                                                                                                                                  >  1:279643/1‑139 (MQ=255)

    aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAg                                                                                                                                  >  2:290380/1‑139 (MQ=255)

         tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCata                                                                                                                             <  2:149981/139‑1 (MQ=255)

          aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  2:298940/139‑1 (MQ=255)

          aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:321090/139‑1 (MQ=255)

           cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            >  2:290916/1‑138 (MQ=255)

           cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:290916/138‑1 (MQ=255)

                  tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAag                                                                                                                    >  2:538278/1‑139 (MQ=255)

                         cATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGt                                                                                                             <  2:490810/139‑1 (MQ=255)

                          aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTg                                                                                                            >  1:455058/1‑139 (MQ=255)

                                gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCg                                                                                                      >  2:317644/1‑139 (MQ=255)

                                 aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCa                                                                                                                               <  1:287223/113‑1 (MQ=255)

                                 aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCa                                                                                                                               >  2:287223/1‑113 (MQ=255)

                                    aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTATAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                                  <  2:455058/139‑1 (MQ=255)

                                    aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                                  <  1:538278/139‑1 (MQ=255)

                                                                       aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                               <  1:290380/139‑1 (MQ=255)

                                                                             tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                         >  2:398845/1‑139 (MQ=255)

                                                                                 gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                     <  1:398845/139‑1 (MQ=255)

                                                                                  cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                            <  1:309934/99‑1 (MQ=255)

                                                                                  cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                            >  2:309934/1‑99 (MQ=255)

                                                                                  cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                    >  2:393698/1‑139 (MQ=255)

                                                                                     aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:299702/64‑1 (MQ=255)

                                                                                     aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            >  2:299702/1‑64 (MQ=255)

                                                                                       cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  2:279643/139‑1 (MQ=255)

                                                                                       cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  1:317644/139‑1 (MQ=255)

                                                                                               gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTa                                       >  2:110173/1‑139 (MQ=255)

                                                                                                                      gctgATAATGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                <  1:503510/139‑1 (MQ=255)

                                                                                                                               gATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGAt       <  2:393753/139‑1 (MQ=255)

                                                                                                                                    cGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  1:133460/94‑1 (MQ=255)

                                                                                                                                    cGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt  >  1:601593/1‑139 (MQ=255)

GCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTT  >  NC_000913/2817764‑2818034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A