Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 97 53.3 2484976 94.5% 2348302 111.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,486,736 T→C V206A (GTT→GCT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,7360TC100.0% 219.2 / NA 58V206A (GTT→GCT) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

GGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTT  >  NC_000913/3486606‑3486870
                                                                                                                                  |                                                                                                                                      
ggTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCa                                                                                                                                <  1:595887/139‑1 (MQ=255)
ggTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCa                                                                                                                                <  1:499948/139‑1 (MQ=255)
  tATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACt                                                                                                                              >  1:451674/1‑139 (MQ=255)
  tATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACt                                                                                                                              >  1:307763/1‑139 (MQ=255)
              aaTTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGt                                                                                                                  >  2:694928/1‑139 (MQ=255)
                     cGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTg                                                                                                           >  1:214867/1‑139 (MQ=255)
                        cAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        <  2:478516/139‑1 (MQ=255)
                          ggATATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGGGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:451674/139‑1 (MQ=255)
                          ggAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:606619/139‑1 (MQ=255)
                          ggAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:423775/139‑1 (MQ=255)
                                  gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTgg                                                                                              >  1:1000206/1‑139 (MQ=255)
                                  gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTgg                                                                                              >  1:625098/1‑139 (MQ=255)
                                             ggCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 <  2:217151/93‑1 (MQ=255)
                                             ggCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 >  1:217151/1‑93 (MQ=255)
                                                 gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  1:878839/1‑139 (MQ=255)
                                                 gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  1:701556/1‑139 (MQ=255)
                                                 gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  2:999887/1‑139 (MQ=255)
                                                    ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      >  2:1105518/1‑113 (MQ=255)
                                                    ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  1:1105518/113‑1 (MQ=255)
                                                    ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgcg                                                                                       <  1:156492/128‑1 (MQ=255)
                                                    ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgcg                                                                                       >  2:156492/1‑128 (MQ=255)
                                                            aCCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCTGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt                                                                    <  2:878839/139‑1 (MQ=255)
                                                            aCCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt                                                                    <  1:1167981/139‑1 (MQ=255)
                                                                tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                              <  1:1189445/125‑1 (MQ=255)
                                                                tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                              >  2:1189445/1‑125 (MQ=255)
                                                                tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTgg                                                                >  2:611656/1‑139 (MQ=255)
                                                                 gggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgc                                                               <  1:541508/139‑1 (MQ=255)
                                                                  ggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                              <  1:611656/139‑1 (MQ=255)
                                                                  ggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                              <  2:701556/139‑1 (MQ=255)
                                                                     cGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAg                                                           <  2:848808/139‑1 (MQ=255)
                                                                     cGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAg                                                           <  2:625098/139‑1 (MQ=255)
                                                                       cATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGAc                                                         >  1:560653/1‑139 (MQ=255)
                                                                          tCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGa                                                      >  1:335489/1‑139 (MQ=255)
                                                                                  tGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 >  2:826665/1‑56 (MQ=255)
                                                                                  tGCTGGAAGATCAGAACCTGATCTCCCCACACGGTACAACCATCGTCGCTTACGGc                                                                                                                                 <  1:826665/56‑1 (MQ=255)
                                                                                   gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  1:127756/139‑1 (MQ=255)
                                                                                   gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:307763/139‑1 (MQ=255)
                                                                                   gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  2:387536/1‑139 (MQ=255)
                                                                                    cTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAccc                                            >  1:21405/1‑139 (MQ=255)
                                                                                           aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:622310/1‑131 (MQ=255)
                                                                                           aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:622310/131‑1 (MQ=255)
                                                                                             cAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCaa                                   >  2:841876/1‑139 (MQ=255)
                                                                                                aaCCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTa                                <  1:694928/139‑1 (MQ=255)
                                                                                                 aCCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTAt                               <  2:560653/139‑1 (MQ=255)
                                                                                                  ccTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATg                              <  1:999887/139‑1 (MQ=255)
                                                                                                  ccTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATg                              <  2:736100/139‑1 (MQ=255)
                                                                                                     gATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:744894/121‑1 (MQ=255)
                                                                                                     gATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:744894/1‑121 (MQ=255)
                                                                                                          ccGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGa                      >  2:1037099/1‑139 (MQ=255)
                                                                                                                acGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACg                >  1:225282/1‑139 (MQ=255)
                                                                                                                acGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACg                <  2:214867/139‑1 (MQ=255)
                                                                                                                  ggTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCt              <  2:225282/139‑1 (MQ=255)
                                                                                                                     aaaaCCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTGCTCCGATGTGCCGCAGACTGATTTATCACCCCGATATCAACTACGCACTTCGACAAACGCTGGt           >  2:773897/1‑139 (MQ=255)
                                                                                                                          cATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        >  1:333812/1‑41 (MQ=255)
                                                                                                                          cATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        <  2:333812/41‑1 (MQ=255)
                                                                                                                            tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          <  1:526886/133‑1 (MQ=255)
                                                                                                                            tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          >  2:526886/1‑133 (MQ=255)
                                                                                                                            tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          >  1:987088/1‑133 (MQ=255)
                                                                                                                            tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          <  2:987088/133‑1 (MQ=255)
                                                                                                                             cgtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:278289/97‑1 (MQ=255)
                                                                                                                             cgtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:278289/1‑97 (MQ=255)
                                                                                                                              gtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGttt  >  1:200902/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTT  >  NC_000913/3486606‑3486870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A