Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,017	C→T	G35S (GGT→AGT)	gyrB ←	DNA gyrase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,017	0	C	T	100.0%	211.6 / NA	60	G35S (GGT→AGT)	gyrB	DNA gyrase, subunit B
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/29); total (31/29)

GTCGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879881‑3880150

gTCGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTgg                                                                                                                                     >  1:165681/1‑139 (MQ=255)

               ccGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgtgt                                                                                                                      <  1:580562/139‑1 (MQ=255)

               ccGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgtgt                                                                                                                      <  1:31122/139‑1 (MQ=255)

                    aTCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  1:334609/139‑1 (MQ=255)

                               agacagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATAcc                                                                                                      >  1:859088/1‑139 (MQ=255)

                                   agagTTATCGGCGTTAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                  <  2:165681/139‑1 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgt                                                                                                                        <  2:273898/116‑1 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgt                                                                                                                        >  1:273898/1‑116 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    >  2:939741/1‑120 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    <  1:939741/120‑1 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  2:232067/1‑123 (MQ=255)

                                    gagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  1:232067/123‑1 (MQ=255)

                                         aTCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTa                                                                                            >  1:95784/1‑139 (MQ=255)

                                          tCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTAc                                                                                           >  2:92898/1‑139 (MQ=255)

                                                 gAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGc                                                                                    >  2:543157/1‑139 (MQ=255)

                                                        gACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  2:1230088/139‑1 (MQ=255)

                                                         aCGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                  <  2:1145986/117‑1 (MQ=255)

                                                         aCGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                  >  1:1145986/1‑117 (MQ=255)

                                                                tttCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAg                                                                     <  1:92898/139‑1 (MQ=255)

                                                                tttCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAg                                                                     <  2:95784/139‑1 (MQ=255)

                                                                   cTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGAc                                                                  <  1:1079942/139‑1 (MQ=255)

                                                                   cTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGAc                                                                  >  1:1019598/1‑139 (MQ=255)

                                                                   cTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGAc                                                                  >  1:194257/1‑139 (MQ=255)

                                                                     ttACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    >  2:12538/1‑87 (MQ=255)

                                                                     ttACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    <  1:12538/87‑1 (MQ=255)

                                                                     ttACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACtt                                                                >  2:186278/1‑139 (MQ=255)

                                                                         aGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgtgt                                                                                                                      <  2:629873/81‑1 (MQ=255)

                                                                         aGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCgtgt                                                                                                                      >  1:629873/1‑81 (MQ=255)

                                                                           tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATAc                                                          >  2:1120217/1‑139 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGcc                                                                                                                                 <  1:1141506/66‑1 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGcc                                                                                                                                 >  2:1141506/1‑66 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAc                                                                                       <  2:955456/108‑1 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAc                                                                                       >  1:955456/1‑108 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  2:61141/1‑139 (MQ=255)

                                                                             aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  1:150380/1‑139 (MQ=255)

                                                                              cccGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAcc                                                                                      <  2:270988/108‑1 (MQ=255)

                                                                              cccGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAcc                                                                                      >  1:270988/1‑108 (MQ=255)

                                                                               ccGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:150380/139‑1 (MQ=255)

                                                                               ccGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  1:186278/139‑1 (MQ=255)

                                                                                      cgcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCa                                               >  1:144493/1‑139 (MQ=255)

                                                                                       gcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCAt                                              <  2:194257/139‑1 (MQ=255)

                                                                                             tcgATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAAt                                        <  2:1019598/139‑1 (MQ=255)

                                                                                              cgATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAACAAtt                                       <  2:1079699/139‑1 (MQ=255)

                                                                                                                ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                     <  2:859088/139‑1 (MQ=255)

                                                                                                                ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                     <  1:543157/139‑1 (MQ=255)

                                                                                                                  tCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                   >  1:296136/1‑139 (MQ=255)

                                                                                                                   cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  2:117057/1‑139 (MQ=255)

                                                                                                                   cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  1:432131/1‑139 (MQ=255)

                                                                                                                   cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  2:158761/1‑139 (MQ=255)

                                                                                                                      aTACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt               <  1:732709/139‑1 (MQ=255)

                                                                                                                          cATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTtata           >  1:1240897/1‑139 (MQ=255)

                                                                                                                           aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  1:644550/36‑1 (MQ=255)

                                                                                                                           aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  2:644550/1‑36 (MQ=255)

                                                                                                                           aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc          <  1:889963/139‑1 (MQ=255)

                                                                                                                           aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc          >  2:889963/1‑139 (MQ=255)

                                                                                                                            tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACt         >  2:407068/1‑139 (MQ=255)

                                                                                                                            tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACt         <  1:407068/139‑1 (MQ=255)

                                                                                                                                   cAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa  >  2:204826/1‑139 (MQ=255)

                                                                                                                                   cAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa  <  1:158761/139‑1 (MQ=255)

                                                                                                                                   cAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa  >  1:629714/1‑139 (MQ=255)

GTCGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879881‑3880150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A