Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,017	C→T	G35S (GGT→AGT)	gyrB ←	DNA gyrase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,017	0	C	T	100.0%	162.0 / NA	48	G35S (GGT→AGT)	gyrB	DNA gyrase, subunit B
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/22); total (26/22)

CGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879883‑3880150

cGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTg                                                                                                                                   >  1:404649/1‑139 (MQ=255)

    aTGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGt                                                                                                                               >  2:152843/1‑139 (MQ=255)

      gCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCa                                                                                                                             >  2:25882/1‑139 (MQ=255)

               gTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    <  1:64063/139‑1 (MQ=255)

                  aTCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:591229/139‑1 (MQ=255)

                       gTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGAtata                                                                                                            >  1:73728/1‑139 (MQ=255)

                             agacagagTTATCGGCGTGAATGGTGATGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATAcc                                                                                                      >  2:275245/1‑139 (MQ=255)

                               acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCg                                                                                                    >  2:400342/1‑139 (MQ=255)

                               acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCg                                                                                                    >  2:353568/1‑139 (MQ=255)

                                 agagTTATCGGCGTGAATGGTGATGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                  <  1:275245/139‑1 (MQ=255)

                                        tCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:73675/1‑117 (MQ=255)

                                        tCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:73675/117‑1 (MQ=255)

                                            cGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAc                                                                                       <  1:152843/139‑1 (MQ=255)

                                                      gACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  1:353568/139‑1 (MQ=255)

                                                          aTAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCtt                                                                         >  2:457203/1‑139 (MQ=255)

                                                                         tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:384510/84‑1 (MQ=255)

                                                                         tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:384510/1‑84 (MQ=255)

                                                                           aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCTCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  1:385413/1‑139 (MQ=255)

                                                                           aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  2:111075/1‑139 (MQ=255)

                                                                              cgcgAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:81018/138‑1 (MQ=255)

                                                                              cgcgAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:81018/1‑138 (MQ=255)

                                                                                     gcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCAt                                              <  1:25882/139‑1 (MQ=255)

                                                                                                            caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGcc                                                                                                                  <  2:24338/48‑1 (MQ=255)

                                                                                                            caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGcc                                                                                                                  >  1:24338/1‑48 (MQ=255)

                                                                                                            caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTGCGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:355914/1‑108 (MQ=255)

                                                                                                            caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:355914/108‑1 (MQ=255)

                                                                                                             accTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  2:136137/84‑1 (MQ=255)

                                                                                                             accTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             >  1:136137/1‑84 (MQ=255)

                                                                                                              ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:242732/1‑47 (MQ=255)

                                                                                                              ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:242732/47‑1 (MQ=255)

                                                                                                              ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             >  1:26842/1‑83 (MQ=255)

                                                                                                              ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  2:26842/83‑1 (MQ=255)

                                                                                                              ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                     <  1:111075/139‑1 (MQ=255)

                                                                                                                 cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:44062/1‑103 (MQ=255)

                                                                                                                 cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:44062/103‑1 (MQ=255)

                                                                                                                 cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  1:485307/1‑139 (MQ=255)

                                                                                                                 cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  2:213348/1‑139 (MQ=255)

                                                                                                                         aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc          <  1:213348/139‑1 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTc                                                                       <  2:337133/77‑1 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTc                                                                       >  1:337133/1‑77 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggag                                                     >  1:265991/1‑95 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggag                                                     <  2:265991/95‑1 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                   >  1:251234/1‑129 (MQ=255)

                                                                                                                          tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                   <  2:251234/129‑1 (MQ=255)

                                                                                                                              gtgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTggg     >  2:609389/1‑139 (MQ=255)

                                                                                                                               tgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGgcgc                                                                                               <  2:514802/48‑1 (MQ=255)

                                                                                                                               tgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGgcgc                                                                                               >  1:514802/1‑48 (MQ=255)

                                                                                                                                 cAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa  <  2:385413/139‑1 (MQ=255)

CGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879883‑3880150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A