Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,110,959	T→C	A183A (GCA→GCG)	tpiA ←	triosephosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,110,959	0	T	C	100.0%	166.8 / NA	45	A183A (GCA→GCG)	tpiA	triosephosphate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21)

GCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTT  >  NC_000913/4110835‑4111089

gCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGt                                                                                                                      >  1:499246/1‑139 (MQ=255)

    aGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCa                                                                                                                  >  1:1006272/1‑139 (MQ=255)

    aGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCa                                                                                                                  >  2:1078529/1‑139 (MQ=255)

      cAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGa                                                                                                                <  1:389796/139‑1 (MQ=255)

          cAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTg                                                                                                            <  2:1006272/139‑1 (MQ=255)

                  cAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTAcc                                                                                                    >  2:299077/1‑139 (MQ=255)

                     cGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGAt                                                                                                 <  2:1210864/139‑1 (MQ=255)

                      gTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGAtt                                                                                                <  2:616116/139‑1 (MQ=255)

                        tAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCACCTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGc                                                                                              <  2:748653/139‑1 (MQ=255)

                          gACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGccc                                                                                            >  2:783716/1‑139 (MQ=255)

                              cgTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATAc                                                                                        <  1:1078529/139‑1 (MQ=255)

                                  tACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGt                                                                                    >  1:24726/1‑139 (MQ=255)

                                            ccgTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAgaga                                                                          >  2:901950/1‑139 (MQ=255)

                                                      tGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCAcc                                                                >  1:287234/1‑139 (MQ=255)

                                                         tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTc                                                             >  2:270820/1‑139 (MQ=255)

                                                         tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTc                                                             >  2:316253/1‑139 (MQ=255)

                                                              tGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            >  1:532917/1‑119 (MQ=255)

                                                              tGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            <  2:532917/119‑1 (MQ=255)

                                                                ttCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGcc                                                      >  2:743950/1‑139 (MQ=255)

                                                                     cGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgcagc                                                 >  1:736808/1‑139 (MQ=255)

                                                                        tGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCAcc                                              <  2:24726/139‑1 (MQ=255)

                                                                         gTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACATCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCAccc                                             <  2:400480/139‑1 (MQ=255)

                                                                          ttAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            >  1:218879/1‑139 (MQ=255)

                                                                            aGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGa                                          <  1:197632/139‑1 (MQ=255)

                                                                             gCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAg                                         <  1:316253/139‑1 (MQ=255)

                                                                             gCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAg                                         <  1:270820/139‑1 (MQ=255)

                                                                                    cTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAg                                  >  1:516048/1‑139 (MQ=255)

                                                                                              gtgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCg                        >  1:1024061/1‑139 (MQ=255)

                                                                                               tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGa                       >  1:130979/1‑139 (MQ=255)

                                                                                               tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATT‑CCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                >  2:1106155/1‑129 (MQ=255)

                                                                                               tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATT‑CCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                <  1:1106155/129‑1 (MQ=255)

                                                                                                       gATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                            <  1:924590/94‑1 (MQ=255)

                                                                                                       gATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                            >  2:924590/1‑94 (MQ=255)

                                                                                                          gAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcg            <  2:499246/139‑1 (MQ=255)

                                                                                                           aaTTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcgc           >  1:33178/1‑139 (MQ=255)

                                                                                                           aaTTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcgc           >  1:177455/1‑139 (MQ=255)

                                                                                                             tttGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         <  2:736808/139‑1 (MQ=255)

                                                                                                              ttGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaaa        <  1:299077/139‑1 (MQ=255)

                                                                                                                gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            <  2:954704/69‑1 (MQ=255)

                                                                                                                gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            >  1:954704/1‑69 (MQ=255)

                                                                                                                gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         <  2:104311/136‑1 (MQ=255)

                                                                                                                gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         >  1:104311/1‑136 (MQ=255)

                                                                                                                    aCAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAActtctt  <  1:955471/139‑1 (MQ=255)

                                                                                                                        ccTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            >  2:288719/1‑93 (MQ=255)

                                                                                                                        ccTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            <  1:288719/93‑1 (MQ=255)

GCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTT  >  NC_000913/4110835‑4111089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A