Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,604	C→T	R227C (CGC→TGC)	zraS →	sensory histidine kinase in two‑component regulatory system with ZraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,604	0	C	T	100.0%	154.7 / NA	46	R227C (CGC→TGC)	zraS	sensory histidine kinase in two‑component regulatory system with ZraR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/24); total (22/24)

TTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATA  >  NC_000913/4202469‑4202731

tttATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt                                                                                                                              <  1:1117847/139‑1 (MQ=255)

    tCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgcg                                                                                                                          <  1:131239/139‑1 (MQ=255)

    tCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgcg                                                                                                                          <  1:894665/139‑1 (MQ=255)

    tCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgcg                                                                                                                          <  1:39421/139‑1 (MQ=255)

        cGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAg                                                                                                                      >  1:528354/1‑139 (MQ=255)

          ttGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCt                                                                                                                    >  2:705521/1‑139 (MQ=255)

                ccAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACa                                                                                                              <  2:291336/139‑1 (MQ=255)

                ccAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACa                                                                                                              <  2:528354/139‑1 (MQ=255)

                  aGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAg                                                                                                            <  2:981467/139‑1 (MQ=255)

                        cTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgcg                                                                                                                          <  1:311701/119‑1 (MQ=255)

                        cTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgcg                                                                                                                          >  2:311701/1‑119 (MQ=255)

                               cAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAgcg                                                                                               <  2:971233/139‑1 (MQ=255)

                                aaCCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt                                                                                                                              <  1:462053/107‑1 (MQ=255)

                                aaCCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt                                                                                                                              >  2:462053/1‑107 (MQ=255)

                                aaCCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAgcgc                                                                                              >  2:191575/1‑139 (MQ=255)

                                      gagTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCAGGCGACGGTGTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAagag                                                                                        >  2:761941/1‑139 (MQ=255)

                                           gggAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCt                                                                                   >  2:359788/1‑139 (MQ=255)

                                                 aGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGgc                                                                             >  2:448754/1‑139 (MQ=255)

                                                             tGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCACGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCt                                                                 >  2:958301/1‑139 (MQ=255)

                                                                ttATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTAc                                                                                                               <  2:88093/90‑1 (MQ=255)

                                                                ttATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTAc                                                                                                               >  1:88093/1‑90 (MQ=255)

                                                                  aTCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCgg                                                            >  2:1164136/1‑139 (MQ=255)

                                                                   tCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGc                                                           <  2:655900/139‑1 (MQ=255)

                                                                   tCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGc                                                           <  1:812197/139‑1 (MQ=255)

                                                                    ccTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCa                                                          >  1:37333/1‑139 (MQ=255)

                                                                     ctctTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAg                                                         >  1:466944/1‑139 (MQ=255)

                                                                        ttCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCg                                                      <  1:1024889/139‑1 (MQ=255)

                                                                        ttCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCg                                                      >  2:180905/1‑139 (MQ=255)

                                                                        ttCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCAGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGct                                                      <  1:761941/139‑2 (MQ=255)

                                                                         tCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGt                                                     >  2:668092/1‑139 (MQ=255)

                                                                             ccTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGcc                                                 >  2:367676/1‑139 (MQ=255)

                                                                               tGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACa                                                                                                              <  1:106256/76‑1 (MQ=255)

                                                                               tGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACa                                                                                                              >  2:106256/1‑76 (MQ=255)

                                                                                        tCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCg                                      <  1:705521/139‑1 (MQ=255)

                                                                                          ttGCTGGCAAGCGTATTGTCAGTCTTCGGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTa                                    <  1:191575/139‑1 (MQ=255)

                                                                                           tGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAaga                                                                                         <  2:371262/85‑1 (MQ=255)

                                                                                           tGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAaga                                                                                         >  1:371262/1‑85 (MQ=255)

                                                                                                  aaGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCg                                      >  1:248756/1‑129 (MQ=255)

                                                                                                  aaGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCg                                      <  2:248756/129‑1 (MQ=255)

                                                                                                    gCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGTGGCAGGCGTTg                                                   >  1:1025501/1‑114 (MQ=255)

                                                                                                    gCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGTGGCAGGCGTTg                                                   <  2:1025501/114‑1 (MQ=255)

                                                                                                       tATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTc                       <  2:1226435/139‑1 (MQ=255)

                                                                                                                   tctGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGatgaaatg                                                                                                    >  2:619730/1‑50 (MQ=255)

                                                                                                                   tctGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGatgaaatg                                                                                                    <  1:619730/50‑1 (MQ=255)

                                                                                                                       gTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCg       <  2:466944/139‑1 (MQ=255)

                                                                                                                            gccgcTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATa  >  1:53025/1‑139 (MQ=255)

TTTATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCACTTTCCTCGATTAAAGGACTGGCGAAATA  >  NC_000913/4202469‑4202731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A