Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,202,604 |
C→T |
R227C (CGC→TGC) |
zraS → |
sensory histidine kinase in two‑component regulatory system with ZraR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,202,604 | 0 | C | T | 100.0%
| 37.2
/ NA
| 12 | R227C (CGC→TGC) | zraS | sensory histidine kinase in two‑component regulatory system with ZraR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/5); total (7/5) |
ATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCA > NC_000913/4202472‑4202702
|
aTCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCACAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgc > 1:48004/1‑139 (MQ=255)
tGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt < 1:15308/119‑1 (MQ=255)
tGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt > 2:15308/1‑119 (MQ=255)
ttGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGatgaaatgaa < 2:256951/139‑1 (MQ=255)
aCCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCa > 2:438076/1‑139 (MQ=255)
cccAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCaa > 2:375376/1‑139 (MQ=255)
gagTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAagag > 2:88820/1‑139 (MQ=255)
ggAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCtg < 1:88820/139‑1 (MQ=255)
ccTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCa > 1:603692/1‑139 (MQ=255)
tGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCa > 2:67855/1‑139 (MQ=255)
gCTGGCAAGCGTATTGTCATTCTTCTGTTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGACAGGCGTTGCCCACGAAATCCGTAAc < 1:438076/139‑1 (MQ=255)
ggCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCa < 2:603692/139‑1 (MQ=255)
|
ATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCA > NC_000913/4202472‑4202702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
