Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 97 53.3 2484976 94.5% 2348302 111.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,305,268 A→G Y444H (TAT→CAT)  yjcS ← metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,305,2680AG100.0% 68.5 / NA 19Y444H (TAT→CAT) yjcSmetallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

CCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGC  >  NC_000913/4305142‑4305392
                                                                                                                              |                                                                                                                            
ccTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGtt                                                                                                                  <  1:801736/139‑1 (MQ=255)
    tttCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAgg                                                                                                                    >  2:738863/1‑133 (MQ=255)
    tttCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAgg                                                                                                                    <  1:738863/133‑1 (MQ=255)
                             tAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGAt                                                                                     <  1:1091182/139‑1 (MQ=255)
                                       gcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCggg                                                                                                           <  2:831135/107‑1 (MQ=255)
                                       gcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCggg                                                                                                           >  1:831135/1‑107 (MQ=255)
                                                  tcttGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCggg                                                                >  1:917602/1‑139 (MQ=255)
                                                             gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGTTg                                                     >  2:1161851/1‑139 (MQ=255)
                                                             gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTg                                                     >  2:481407/1‑139 (MQ=255)
                                                              ttGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGa                                                    <  2:448083/139‑1 (MQ=255)
                                                                 atgaCACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAg                                                 >  1:656114/1‑139 (MQ=255)
                                                                 atgaCACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGAAAg                                                 >  2:9670/1‑139 (MQ=255)
                                                                        gggCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTa                                          >  1:225922/1‑139 (MQ=255)
                                                                                        cgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGcc                                                                                          <  2:1138661/75‑1 (MQ=255)
                                                                                        cgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGcc                                                                                          >  1:1138661/1‑75 (MQ=255)
                                                                                                     cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAg                                                                                   >  2:473678/1‑69 (MQ=255)
                                                                                                     cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAg                                                                                   <  1:473678/69‑1 (MQ=255)
                                                                                                     cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCaa             <  1:481407/139‑1 (MQ=255)
                                                                                                                ctcCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcggc  >  1:390979/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                            
CCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGC  >  NC_000913/4305142‑4305392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A