Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
543,768 |
A→G |
T93T (ACT→ACC) |
allE ← |
S‑ureidoglycine aminohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 543,768 | 0 | A | G | 100.0%
| 110.1
/ NA
| 29 | T93T (ACT→ACC) | allE | S‑ureidoglycine aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/15); total (14/15) |
CGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGG > NC_000913/543646‑543890
|
cgcTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAgaga < 2:180671/139‑1 (MQ=255)
cTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATc < 2:271217/139‑1 (MQ=255)
aGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGaa > 1:139879/1‑139 (MQ=255)
gCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGa > 1:189624/1‑139 (MQ=255)
ggAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCag < 2:434103/80‑1 (MQ=255)
ggAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCag > 1:434103/1‑80 (MQ=255)
ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCa < 2:605145/132‑1 (MQ=255)
ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCa > 1:605145/1‑132 (MQ=255)
ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCAc < 1:18343/133‑1 (MQ=255)
ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCAc > 2:18343/1‑133 (MQ=255)
ggCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGAt < 2:189624/139‑1 (MQ=255)
cGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGc < 2:97817/139‑1 (MQ=255)
cAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGAt < 2:211134/133‑1 (MQ=255)
cAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGAt > 1:211134/1‑133 (MQ=255)
ccACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATc < 1:200761/60‑1 (MQ=255)
ccACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATc > 2:200761/1‑60 (MQ=255)
aCCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATca > 1:761860/1‑139 (MQ=255)
tCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATcaacaa < 2:139879/139‑1 (MQ=255)
gCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACaaaa < 2:71759/139‑1 (MQ=255)
cTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAag > 2:459428/1‑139 (MQ=255)
aGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGc > 1:140682/1‑75 (MQ=255)
aGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGc < 2:140682/75‑1 (MQ=255)
ggCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGAcc < 2:196312/118‑1 (MQ=255)
ggCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGAcc > 1:196312/1‑118 (MQ=255)
ttGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCg < 1:783186/69‑1 (MQ=255)
ttGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCg > 2:783186/1‑69 (MQ=255)
tGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGcc < 2:273541/73‑1 (MQ=255)
tGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGcc > 1:273541/1‑73 (MQ=255)
tGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTgg > 2:323905/1‑139 (MQ=255)
|
CGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGG > NC_000913/543646‑543890
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
