Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	543,768	A→G	T93T (ACT→ACC)	allE ←	S‑ureidoglycine aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	543,768	0	A	G	100.0%	129.6 / NA	34	T93T (ACT→ACC)	allE	S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/21); total (13/21)

CCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGG  >  NC_000913/543632‑543890

ccGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGt                                                                                                                          <  2:489713/139‑1 (MQ=255)

ccGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGt                                                                                                                          <  2:900486/139‑1 (MQ=255)

        tAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTc                                                                                                                  <  2:751422/139‑1 (MQ=255)

           cggcgCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCag                                                                                                               <  2:123776/139‑1 (MQ=255)

            ggcgCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCaga                                                                                                              <  1:1008937/139‑1 (MQ=255)

              cgcTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAgaga                                                                                                            <  2:456355/139‑1 (MQ=255)

                          aaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGa                                                                                                <  2:876782/139‑1 (MQ=255)

                                      tCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGc                                                                                    <  1:89729/139‑1 (MQ=255)

                                                 ggCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTcc                                                                                                                 >  2:939834/1‑99 (MQ=255)

                                                 ggCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTcc                                                                                                                 <  1:939834/99‑1 (MQ=255)

                                                 ggCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCg                                                                         >  1:644303/1‑139 (MQ=255)

                                                             cGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttgg                                                             >  1:995990/1‑139 (MQ=255)

                                                                cATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttggttg                                                          >  1:832943/1‑139 (MQ=255)

                                                                        aGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGtt                                                  <  2:592283/139‑1 (MQ=255)

                                                                          ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAgaga                                                                                                            <  1:383228/79‑1 (MQ=255)

                                                                          ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAgaga                                                                                                            >  2:383228/1‑79 (MQ=255)

                                                                          ccTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGtttt                                                >  2:577350/1‑139 (MQ=255)

                                                                           cTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTcc                                                                                                                 <  2:342988/73‑1 (MQ=255)

                                                                           cTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTcc                                                                                                                 >  1:342988/1‑73 (MQ=255)

                                                                               cGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGc                                           <  1:577350/139‑1 (MQ=255)

                                                                               cGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGc                                           <  2:807496/139‑1 (MQ=255)

                                                                                      tAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAg                                                                                                  <  2:901069/77‑1 (MQ=255)

                                                                                      tAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAg                                                                                                  >  1:901069/1‑77 (MQ=255)

                                                                                        aaGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGAcc                                  <  2:644303/139‑1 (MQ=255)

                                                                                              gCCACCTTCGCTTAAGGCAAATGTTTTGTCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATaa                            >  2:248321/1‑139 (MQ=255)

                                                                                               ccACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttggt                                                            >  2:6969/1‑106 (MQ=255)

                                                                                               ccACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttggt                                                            <  1:6969/106‑1 (MQ=255)

                                                                                                    ttCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGaa                                                                       >  1:147113/1‑90 (MQ=255)

                                                                                                    ttCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGaa                                                                       <  2:147113/90‑1 (MQ=255)

                                                                                                                  aTGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGaa                                                                                               >  1:928536/1‑52 (MQ=255)

                                                                                                                  aTGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGaa                                                                                               <  2:912495/52‑1 (MQ=255)

                                                                                                                  aTGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGaa                                                                                               <  2:928536/52‑1 (MQ=255)

                                                                                                                  aTGTTTTGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGaa                                                                                               >  1:912495/1‑52 (MQ=255)

                                                                                                                        tGCCTTCGGCTTTGGCGGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTgg  <  2:995990/139‑1 (MQ=255)

CCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGG  >  NC_000913/543632‑543890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A