Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
600,470 |
C→T |
A586V (GCG→GTG) |
cusA → |
copper/silver efflux system, membrane component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 600,470 | 0 | C | T | 100.0%
| 84.5
/ NA
| 25 | A586V (GCG→GTG) | cusA | copper/silver efflux system, membrane component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/13); total (12/13) |
TGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTA > NC_000913/600353‑600604
|
tGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGa < 2:378656/139‑1 (MQ=255)
cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa < 1:353144/139‑1 (MQ=255)
cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa < 1:770944/139‑1 (MQ=255)
cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa < 1:495216/139‑1 (MQ=255)
ggCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTCTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg < 2:425320/139‑1 (MQ=255)
gCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt > 1:771961/1‑112 (MQ=255)
gCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt < 2:771961/112‑1 (MQ=255)
gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa < 2:386312/78‑1 (MQ=255)
gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa > 1:386312/1‑78 (MQ=255)
gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAAc > 1:406830/1‑139 (MQ=255)
tGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGc > 1:153227/1‑139 (MQ=255)
gCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCt < 2:357008/139‑1 (MQ=255)
gCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCt > 1:357008/1‑139 (MQ=255)
cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg > 1:738791/1‑62 (MQ=255)
cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg < 2:738791/62‑1 (MQ=255)
cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg > 1:684070/1‑73 (MQ=255)
cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg < 2:684070/73‑1 (MQ=255)
cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGt < 2:51730/73‑1 (MQ=255)
cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGt > 1:51730/1‑73 (MQ=255)
cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGt < 1:625471/82‑1 (MQ=255)
cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGt > 2:625471/1‑82 (MQ=255)
cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCt > 1:16418/1‑139 (MQ=255)
cTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGgaga < 2:16418/139‑1 (MQ=255)
agcagAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATc > 1:104271/1‑139 (MQ=255)
agGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCt > 2:456081/1‑139 (MQ=255)
gcggtgAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTa < 2:104271/139‑1 (MQ=255)
|
TGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTA > NC_000913/600353‑600604
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
