Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,829,493	G→A	S149S (TCG→TCA)	srlR →	sorbitol‑inducible srl operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,829,493	0	G	A	100.0%	123.3 / NA	37	S149S (TCG→TCA)	srlR	sorbitol‑inducible srl operon transcriptional repressor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/20); total (17/20)

GCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829373‑2829628

gCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  1:611858/139‑1 (MQ=255)

 ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             <  1:177326/132‑1 (MQ=255)

 ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             >  2:177326/1‑132 (MQ=255)

           ttAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGaa                                                                                                            >  1:253486/1‑139 (MQ=255)

                  cATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTc                                                                                                     <  1:623745/139‑1 (MQ=255)

                   aTCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACTAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTGCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  1:582560/139‑1 (MQ=255)

                    tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGa                                                                                                   >  1:56466/1‑139 (MQ=255)

                     cACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAg                                                                                                  <  2:253486/139‑1 (MQ=255)

                      aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  2:278741/1‑136 (MQ=255)

                      aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  1:278741/136‑1 (MQ=255)

                               aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  1:515708/1‑127 (MQ=255)

                               aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  2:515708/127‑1 (MQ=255)

                                    cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  1:489578/1‑122 (MQ=255)

                                    cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  2:489578/122‑1 (MQ=255)

                                    cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                                                   >  2:508463/1‑139 (MQ=255)

                                    cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                                                   >  1:32565/1‑139 (MQ=255)

                                     aGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCTAGCATTTCACCTTCGATa                                                                                  <  2:527577/139‑1 (MQ=255)

                                       ccTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaa                                                                                <  1:85873/139‑1 (MQ=255)

                                        cTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt                                                                               >  1:669474/1‑139 (MQ=255)

                                        cTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt                                                                               <  2:32565/139‑1 (MQ=255)

                                         tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaact                                                                              >  2:286400/1‑137 (MQ=255)

                                                 gTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCt                                                                                        <  1:255921/121‑1 (MQ=255)

                                                 gTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCt                                                                                        >  2:255921/1‑121 (MQ=255)

                                                     aTGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAc                                                                  >  2:96313/1‑139 (MQ=255)

                                                          cTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  1:536151/81‑1 (MQ=255)

                                                          cTATCCGAACTGGATAACGAACAAACTATCCTGATGCAAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       >  2:536151/1‑81 (MQ=255)

                                                           tATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACgg                                                            >  2:35535/1‑139 (MQ=255)

                                                             tCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCa                                                          >  1:598234/1‑139 (MQ=255)

                                                                       aTAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCaa                                                >  1:768410/1‑139 (MQ=255)

                                                                       aTAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCaa                                                >  1:145788/1‑139 (MQ=255)

                                                                           cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCg                                            <  2:768410/139‑1 (MQ=255)

                                                                            gAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCgg                                           <  1:96313/139‑1 (MQ=255)

                                                                                   cTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac                                    <  2:617151/139‑1 (MQ=255)

                                                                                             gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  2:669474/139‑1 (MQ=255)

                                                                                             gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACAGACATCTGCCAGTGAATGCCTTCGATCATTTCACCTGCGAGAAATTGTTTATGGGCAGCGACGGGATCGATCTTAATGCGGGCGTAACCACCTTTAAc                          <  1:142632/139‑1 (MQ=255)

                                                                                                                     aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCa  <  1:24675/139‑1 (MQ=255)

                                                                                                                     aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCa  <  2:56466/139‑1 (MQ=255)

GCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829373‑2829628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A