Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,194,362 |
(G)6→7 |
coding (1499/1662 nt) |
yqiK → |
PHB family membrane protein, function unknown |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,194,356 | 1 | . | G | 100.0%
| 86.2
/ NA
| 23 | V498G (GTG→GGG) | yqiK | PHB family membrane protein, function unknown |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (11/12); total (11/12) |
GCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCAT > NC_000913/3194223‑3194475
|
gCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑ggggg > 1:696282/1‑139 (MQ=255)
ggCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCt > 1:422044/1‑139 (MQ=255)
gcctgcGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGa < 2:422044/139‑1 (MQ=255)
cctgcGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGaa < 2:696282/139‑1 (MQ=255)
cctgcGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGaa < 2:452257/139‑1 (MQ=255)
gagaAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCa < 1:193142/139‑1 (MQ=255)
aaTCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCg < 1:556358/139‑1 (MQ=255)
aaTCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCg < 1:62706/139‑1 (MQ=255)
aTCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGc > 2:660186/1‑139 (MQ=255)
aTCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGc > 2:412137/1‑139 (MQ=255)
gTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGctctc > 2:121380/1‑139 (MQ=255)
aGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑ggg > 1:437479/1‑94 (MQ=255)
aGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑ggg < 2:437479/94‑1 (MQ=255)
tCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc > 2:450840/1‑139 (MQ=255)
ggTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTg > 2:633568/1‑139 (MQ=255)
attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGc > 2:748162/1‑99 (MQ=255)
attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGc < 1:748162/99‑1 (MQ=255)
attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTg < 1:633568/139‑1 (MQ=255)
attCAGGTCGATGGTCTGAATCATGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTg > 2:434883/1‑139 (MQ=255)
gTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAAt > 1:638757/1‑139 (MQ=255)
gTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTg < 2:638757/139‑1 (MQ=255)
cGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCa < 1:681127/139‑1 (MQ=255)
tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg < 1:434883/139‑1 (MQ=255)
tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg > 2:698374/1‑139 (MQ=255)
tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg > 1:103868/1‑139 (MQ=255)
aTGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCAt < 2:370081/139‑1 (MQ=255)
|
GCCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCAT > NC_000913/3194223‑3194475
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
