Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,538	A→G	S270P (TCT→CCT)	tdh ←	L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,538	0	A	G	100.0%	159.8 / NA	42	S270P (TCT→CCT)	tdh	L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/20); total (22/20)

ATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT  >  NC_000913/3790404‑3790669

aTGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCata                                                                                                                                 >  2:531723/1‑139 (MQ=255)

     tAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGa                                                                                                                            <  2:428113/139‑1 (MQ=255)

         gATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGAcggc                                                                                                                        >  2:81723/1‑139 (MQ=255)

            cGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGa                                                                                                                     <  1:531723/139‑1 (MQ=255)

             ggCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGaa                                                                                                                    >  1:461285/1‑139 (MQ=255)

              gCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAAt                                                                                                                   >  2:483095/1‑139 (MQ=255)

               cGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATa                                                                                                                  >  1:255962/1‑139 (MQ=255)

                 aaaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATAcc                                                                                                                <  2:375141/139‑1 (MQ=255)

                    gATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAg                                                                                                             <  2:392506/139‑1 (MQ=255)

                       cGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCAt                                                                                                          <  2:102768/139‑1 (MQ=255)

                                  tGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               <  2:461285/139‑1 (MQ=255)

                                            ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGAtt                                                                                     >  1:546035/1‑139 (MQ=255)

                                              gccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   >  2:296913/1‑139 (MQ=255)

                                                        aCCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAg                                                                         <  2:279922/139‑1 (MQ=255)

                                                            ggTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATg                                                                     >  2:195129/1‑139 (MQ=255)

                                                                        ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc                                                                        <  1:331769/124‑1 (MQ=255)

                                                                        ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc                                                                        >  2:331769/1‑124 (MQ=255)

                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   >  1:783370/1‑103 (MQ=255)

                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   <  2:783370/103‑1 (MQ=255)

                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATg                                                                                 >  2:163306/1‑105 (MQ=255)

                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATg                                                                                 <  1:163306/105‑1 (MQ=255)

                                                                                      tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:81723/139‑1 (MQ=255)

                                                                                      tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:483095/139‑1 (MQ=255)

                                                                                         cTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAt                                        >  2:352382/1‑139 (MQ=255)

                                                                                            tAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTc                                     >  2:279703/1‑139 (MQ=255)

                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               <  1:500542/80‑1 (MQ=255)

                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               >  2:500542/1‑80 (MQ=255)

                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           >  2:703000/1‑132 (MQ=255)

                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:703000/132‑1 (MQ=255)

                                                                                                      gCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGa                                                               >  1:65795/1‑103 (MQ=255)

                                                                                                      gCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGa                                                               <  2:65795/103‑1 (MQ=255)

                                                                                                           tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGAcc                               <  1:568580/130‑1 (MQ=255)

                                                                                                           tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGAcc                               >  2:568580/1‑130 (MQ=255)

                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  2:360435/1‑139 (MQ=255)

                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  1:370345/1‑139 (MQ=255)

                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  2:302349/1‑139 (MQ=255)

                                                                                                                   aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg              <  1:306127/139‑1 (MQ=255)

                                                                                                                   aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg              <  1:302349/139‑1 (MQ=255)

                                                                                                                    cTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGt             <  1:785090/139‑1 (MQ=255)

                                                                                                                    cTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGt             <  1:195129/139‑1 (MQ=255)

                                                                                                                               tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGATATCAAAACCTTCGGTCATGCCTAACt  >  1:68797/1‑139 (MQ=255)

                                                                                                                               tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACt  >  2:422323/1‑139 (MQ=255)

ATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT  >  NC_000913/3790404‑3790669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A