Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,624,129 |
C→T |
F362F (TTC→TTT) |
ppk → |
polyphosphate kinase, component of RNA degradosome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,624,129 | 0 | C | T | 100.0%
| 55.8
/ NA
| 17 | F362F (TTC→TTT) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/7); total (10/7) |
CGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCG > NC_000913/2623995‑2624255
|
cgTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGAcc > 2:231803/1‑139 (MQ=255)
aGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAAc > 2:265684/1‑139 (MQ=255)
gCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTCCTCTACTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGt > 1:305227/1‑139 (MQ=255)
cAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTaaaa > 1:208711/1‑122 (MQ=255)
cAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTaaaa < 2:208711/122‑1 (MQ=255)
gATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCga < 2:349029/139‑1 (MQ=255)
tGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCa < 2:402053/139‑1 (MQ=255)
ctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcgcc < 1:265684/139‑1 (MQ=255)
tattatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAACTTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCAcgccg > 1:139664/1‑139 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCg > 1:4318/1‑100 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCg < 2:4318/100‑1 (MQ=255)
atCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGAAGATCCACGCCGcaca < 2:305227/139‑1 (MQ=255)
tGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGt > 1:244047/1‑130 (MQ=255)
tGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGt < 2:244047/130‑1 (MQ=255)
tGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTc > 2:218912/1‑139 (MQ=255)
ctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc > 1:265363/1‑139 (MQ=255)
tCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAAGATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg > 2:205689/1‑139 (MQ=255)
|
CGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCG > NC_000913/2623995‑2624255
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
