Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
65 |
21.2 |
1002576 |
96.5% |
967485 |
111.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,624,129 |
C→T |
F362F (TTC→TTT) |
ppk → |
polyphosphate kinase, component of RNA degradosome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,624,129 | 0 | C | T | 100.0%
| 90.4
/ NA
| 27 | F362F (TTC→TTT) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/11); total (16/11) |
TTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCG > NC_000913/2624028‑2624262
|
ttCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGAc < 1:387773/105‑1 (MQ=255)
ttCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGAc > 2:387773/1‑105 (MQ=255)
cAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTg > 1:105090/1‑139 (MQ=255)
cATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGc > 2:24093/1‑139 (MQ=255)
aTTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGca > 1:226087/1‑139 (MQ=255)
ttCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGcat > 2:129735/1‑139 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGAtt < 1:21491/76‑1 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGAtt > 2:21491/1‑76 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACt > 1:151780/1‑122 (MQ=255)
ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACt < 2:151780/122‑1 (MQ=255)
tCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt < 1:24093/139‑1 (MQ=255)
tCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt > 1:50086/1‑139 (MQ=255)
aTCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACgg > 2:267737/1‑139 (MQ=255)
acCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGa > 2:367313/1‑139 (MQ=255)
ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaa < 2:226087/139‑1 (MQ=255)
cTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaaa > 1:286495/1‑139 (MQ=255)
ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCAc > 1:42023/1‑93 (MQ=255)
ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCAc < 2:42023/93‑1 (MQ=255)
aGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc < 2:286495/139‑1 (MQ=255)
aGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc < 1:129735/139‑1 (MQ=255)
gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt < 1:496816/117‑1 (MQ=255)
gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt > 2:496816/1‑117 (MQ=255)
gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg > 1:264559/1‑139 (MQ=255)
gctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa > 2:386400/1‑139 (MQ=255)
gctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa < 2:50086/139‑1 (MQ=255)
ctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAAtt > 2:278064/1‑45 (MQ=255)
ctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAAtt < 1:278064/45‑1 (MQ=255)
tcgtttgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcg < 1:386400/139‑1 (MQ=255)
|
TTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCG > NC_000913/2624028‑2624262
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
