Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	66	20.9	912876	97.7%	891879	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,930	G→A	S139S (AGC→AGT)	pncC ←	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,930	0	G	A	100.0%	178.5 / NA	52	S139S (AGC→AGT)	pncC	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/24); total (28/24)

AATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCAC  >  NC_000913/2823806‑2824063

aaTATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCccg                                                                                                                         <  1:76012/139‑1 (MQ=255)

        cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTaa                                                                                                                 >  2:37474/1‑139 (MQ=255)

                     tataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACcacg                                                                                                    >  2:376857/1‑139 (MQ=255)

                              tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           >  2:199376/1‑139 (MQ=255)

                              tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           <  2:89772/139‑1 (MQ=255)

                                   aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAg                                                                                      >  1:219583/1‑139 (MQ=255)

                                    tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAtt                                                                                                                             >  2:237086/1‑99 (MQ=255)

                                    tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAtt                                                                                                                             <  1:237086/99‑1 (MQ=255)

                                    tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGt                                                                                                                   <  2:374391/109‑1 (MQ=255)

                                    tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGt                                                                                                                   >  1:374391/1‑109 (MQ=255)

                                        gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCt                                                                                                            <  2:54272/112‑1 (MQ=255)

                                        gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCt                                                                                                            >  1:54272/1‑112 (MQ=255)

                                        gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAc                                                                                 >  1:365614/1‑139 (MQ=255)

                                         tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAcc                                                                                >  2:391390/1‑139 (MQ=255)

                                          aTCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCa                                                                               >  1:175692/1‑139 (MQ=255)

                                           tCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:370732/1‑139 (MQ=255)

                                           tCAAGTGCTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:440670/1‑139 (MQ=255)

                                            cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:37474/139‑1 (MQ=255)

                                            cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:391390/139‑1 (MQ=255)

                                            cAAGTGCTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:440670/139‑1 (MQ=255)

                                                     tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                              >  2:100332/1‑81 (MQ=255)

                                                     tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                              <  1:100332/81‑1 (MQ=255)

                                                                             tGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              <  1:174539/105‑1 (MQ=255)

                                                                             tGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:174539/1‑105 (MQ=255)

                                                                                     aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCa                                                                                    >  1:106170/1‑91 (MQ=255)

                                                                                     aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccg                                    >  1:75227/1‑139 (MQ=255)

                                                                                     aTACGCAGTAGCCTGACGACGCACCGCATCACGGGCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCa                                                                                    <  2:106170/91‑1 (MQ=255)

                                                                                      tACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccgg                                   <  2:75227/139‑1 (MQ=255)

                                                                                       aCGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccggc                                  >  1:266500/1‑139 (MQ=255)

                                                                                         gCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGa                                >  1:350725/1‑139 (MQ=255)

                                                                                         gCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGa                                <  2:219583/139‑1 (MQ=255)

                                                                                          cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                               <  2:175692/139‑1 (MQ=255)

                                                                                          cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                               <  1:370732/139‑1 (MQ=255)

                                                                                           aGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           <  1:223837/78‑1 (MQ=255)

                                                                                           aGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           >  2:223837/1‑78 (MQ=255)

                                                                                            gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  2:332312/1‑139 (MQ=255)

                                                                                            gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             <  1:199376/139‑1 (MQ=255)

                                                                                            gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  1:353811/1‑139 (MQ=255)

                                                                                            gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  1:360615/1‑139 (MQ=255)

                                                                                             tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                                  <  1:404527/101‑1 (MQ=255)

                                                                                             tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                                  >  2:404527/1‑101 (MQ=255)

                                                                                             tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                <  2:58383/103‑1 (MQ=255)

                                                                                             tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                >  1:58383/1‑103 (MQ=255)

                                                                                              aGCCTTCCGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAATCCAGTCGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa                           <  2:139283/139‑1 (MQ=255)

                                                                                              aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:448938/89‑1 (MQ=255)

                                                                                              aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             >  2:448938/1‑89 (MQ=255)

                                                                                              aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa                           >  1:9029/1‑139 (MQ=255)

                                                                                                ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                >  2:253365/1‑100 (MQ=255)

                                                                                                ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                <  1:253365/100‑1 (MQ=255)

                                                                                                      gacgCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAACAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGa                   <  2:266500/139‑1 (MQ=255)

                                                                                                      gacgCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCACTGGCAAAAGCAAACCAGCCGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGa                   <  2:365614/139‑1 (MQ=255)

                                                                                                            ccGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGc             <  1:332312/139‑1 (MQ=255)

                                                                                                                       tCGCCACTGAAGCATTCCCGCCGGCTAATGCCTTCACCGCGGGCAGTAGTAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCACTCCAGTCGATACCACTAATATACACGGCAAAATCAGCAc  >  2:125483/1‑139 (MQ=255)

AATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCAC  >  NC_000913/2823806‑2824063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A