Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,315,163 |
G→A |
G284G (GGC→GGT) |
infB ← |
translation initiation factor IF‑2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,315,163 | 0 | G | A | 100.0%
| 34.5
/ NA
| 13 | G284G (GGC→GGT) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/7); total (6/7) |
GTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG > NC_000913/3315054‑3315286
|
gttcgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACg > 1:146212/1‑139 (MQ=255)
cgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTATCGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATc > 2:281306/1‑139 (MQ=255)
aTAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGAtt < 2:146212/139‑1 (MQ=255)
tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTg < 1:399272/109‑1 (MQ=255)
tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTg > 2:399272/1‑109 (MQ=255)
tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgt > 1:141362/1‑139 (MQ=255)
ccGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGtgtt < 2:280461/139‑1 (MQ=255)
aCGGCCTGATCAGGCTTCTGTAAGCCTTGCTGCAGCGAAGATCCTTTACGTTTTCCGCCTTTACCACCTCGTACTGTTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg < 1:106071/139‑1 (MQ=255)
tGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAc > 1:207250/1‑139 (MQ=255)
ggAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcc < 2:141362/139‑1 (MQ=255)
aaCCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa > 1:421911/1‑113 (MQ=255)
aaCCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa < 2:421911/113‑1 (MQ=255)
ttttCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCg < 2:446514/139‑1 (MQ=255)
|
GTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG > NC_000913/3315054‑3315286
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
