Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,385,906 |
T→C |
L68P (CTC→CCC) |
yjeO → |
inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,385,906 | 0 | T | C | 100.0%
| 52.3
/ NA
| 15 | L68P (CTC→CCC) | yjeO | inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/8); total (7/8) |
ATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTT > NC_000913/4385794‑4386009
|
atgatTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAc > 1:245523/1‑139 (MQ=255)
atgatTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAc > 2:373930/1‑139 (MQ=255)
attgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg < 2:392004/139‑1 (MQ=255)
ttgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCgg < 1:373930/139‑1 (MQ=255)
ttgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCgg < 2:73488/139‑1 (MQ=255)
atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGc > 1:356155/1‑137 (MQ=255)
atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGc < 2:356155/137‑1 (MQ=255)
cTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg > 1:203392/1‑113 (MQ=255)
cTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg < 2:203392/113‑1 (MQ=255)
gCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGc > 1:95620/1‑139 (MQ=255)
tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg > 1:243363/1‑118 (MQ=255)
tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg < 2:243363/118‑1 (MQ=255)
aagaagACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCtgtg < 1:289440/139‑1 (MQ=255)
gTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATc < 2:95620/139‑1 (MQ=255)
tgtgATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGttt > 1:428062/1‑139 (MQ=255)
|
ATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTT > NC_000913/4385794‑4386009
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
