Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,968,260 |
(T)6→5 |
coding (175/2247 nt) |
ptsP ← |
PEP‑protein phosphotransferase enzyme I, GAF domain containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,968,255 | 0 | T | . | 100.0%
| 116.2
/ NA
| 27 | coding (180/2247 nt) | ptsP | PEP‑protein phosphotransferase enzyme I, GAF domain containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (12/15); total (12/15) |
CGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGG > NC_000913/2968131‑2968389
|
cGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCgg > 2:379588/1‑139 (MQ=255)
gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt > 1:158808/1‑139 (MQ=255)
gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt < 1:63573/139‑1 (MQ=255)
ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCg < 1:100336/139‑1 (MQ=255)
gggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGc < 1:383178/139‑1 (MQ=255)
aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt < 1:369332/139‑1 (MQ=255)
aTGTATTTGAAGCTGGGGGGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCCGCCTGCCAACCAGGCCGACGATCCCTTCTTCATACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCACCCGGGTCGCCAt < 1:119782/139‑1 (MQ=255)
tGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaacaa > 2:178639/1‑139 (MQ=255)
gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt > 1:246978/1‑124 (MQ=255)
gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt < 2:246978/124‑1 (MQ=255)
gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg > 1:172398/1‑139 (MQ=255)
gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg < 1:33062/139‑1 (MQ=255)
tGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg < 2:500716/139‑1 (MQ=255)
tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCg > 1:134461/1‑139 (MQ=255)
tCCGCCAGGCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGAc < 1:312282/139‑1 (MQ=255)
ccAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCAt < 1:52753/139‑1 (MQ=255)
ccTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGt > 1:397104/1‑139 (MQ=255)
atcaAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCa < 2:264253/139‑1 (MQ=255)
gcgAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATAtt > 1:429023/1‑139 (MQ=255)
cgAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt > 2:223165/1‑42 (MQ=255)
cgAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt < 1:223165/42‑1 (MQ=255)
cGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACg < 2:352168/139‑1 (MQ=255)
gTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCAtt < 2:158808/139‑1 (MQ=255)
tGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTc > 1:418988/1‑139 (MQ=255)
ccGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGa > 1:276465/1‑73 (MQ=255)
ccGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGa < 2:276465/73‑1 (MQ=255)
cgTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtgg > 1:468969/1‑139 (MQ=255)
|
CGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGG > NC_000913/2968131‑2968389
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
