Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,868,399 |
C→T |
C304Y (TGT→TAT) |
yidR ← |
DUF3748 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,868,399 | 0 | C | T | 100.0%
| 78.8
/ NA
| 23 | C304Y (TGT→TAT) | yidR | DUF3748 family protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/11); total (12/11) |
AACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTG > NC_000913/3868300‑3868489
|
aaCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGAt < 2:1517/139‑1 (MQ=255)
aCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAAcc < 1:284656/132‑1 (MQ=255)
aCTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAAcc > 2:284656/1‑132 (MQ=255)
gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACTCCGGAt < 2:392640/134‑1 (MQ=255)
gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACTCCGGAt > 1:392640/1‑134 (MQ=255)
gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGcc < 2:193577/139‑1 (MQ=255)
gcgcGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGcc < 1:56625/139‑1 (MQ=255)
cgGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACg > 1:28199/1‑115 (MQ=255)
cgGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACg < 2:28199/115‑1 (MQ=255)
cgccCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGt < 2:83569/139‑1 (MQ=255)
aTAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTaaa > 2:213588/1‑135 (MQ=255)
aTAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTaaa < 1:213588/135‑1 (MQ=255)
aGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCacga > 1:47601/1‑139 (MQ=255)
aGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCacga > 1:424999/1‑139 (MQ=255)
tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGAc < 1:433987/79‑1 (MQ=255)
tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGAc > 2:433987/1‑79 (MQ=255)
tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCgg > 1:47157/1‑139 (MQ=255)
tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCgg > 1:256650/1‑139 (MQ=255)
ccGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTg > 1:369684/1‑139 (MQ=255)
cGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCa < 1:308369/91‑1 (MQ=255)
cGGATTATAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCa > 2:308369/1‑91 (MQ=255)
ggATTATAGCGCGCCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCa < 2:258097/82‑1 (MQ=255)
ggATTATAGCGCGCCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCa > 1:258097/1‑82 (MQ=255)
|
AACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTG > NC_000913/3868300‑3868489
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
