Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,635,400 |
(T)7→6 |
intergenic (‑90/‑121) |
rob ← / → creA |
right oriC‑binding transcriptional activator, AraC family/putative periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,635,394 | 0 | T | . | 100.0%
| 91.2
/ NA
| 22 | intergenic (‑84/‑127) | rob/creA | right oriC‑binding transcriptional activator, AraC family/putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (12/10); total (12/10) |
ATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATA > NC_000913/4635260‑4635528
|
aTGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttt < 2:413789/139‑1 (MQ=255)
ttCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGACTGATACATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACAGCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTtag < 1:190563/139‑1 (MQ=255)
ccagccagATTAAAAGGTCGCGAATAATGGCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGGCATCAGGTAATTGGATAATAGCATTTCTTGCTTTTTTAGCATTCAGTG‑TTTTTTCTtagta < 1:331469/139‑1 (MQ=255)
cgcgAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTGAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTACCATTCAGTGttt > 2:343125/1‑111 (MQ=255)
cgcgAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttt < 1:343125/111‑1 (MQ=255)
gAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGAc < 2:201258/139‑1 (MQ=255)
ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAgtgt > 2:397589/1‑77 (MQ=255)
ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAgtgt < 1:397589/77‑1 (MQ=255)
ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCtt < 1:55717/139‑1 (MQ=255)
cTCATCCTTTCAACAAGTAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTa < 2:348400/139‑1 (MQ=255)
aTCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttttctt > 1:438815/1‑78 (MQ=255)
aTCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttttctt < 2:438815/81‑4 (MQ=255)
cTTTCAACAACGAGCACCTGATATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGTGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTGCTTAATCAat < 1:483109/139‑1 (MQ=255)
ttCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAatat < 2:381311/139‑1 (MQ=255)
ttCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAatat < 2:240915/139‑1 (MQ=255)
tCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATg > 2:184319/1‑139 (MQ=255)
tCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATg > 2:222254/1‑139 (MQ=255)
acaacGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTa > 1:39005/1‑139 (MQ=255)
aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGGCGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTAcc > 1:513454/1‑139 (MQ=255)
aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGtt < 1:103941/132‑1 (MQ=255)
aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGCTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTAcc > 1:11090/1‑139 (MQ=255)
aGCACCTGACATCACGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTGTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTTATCAATATGtt > 2:103941/1‑132 (MQ=255)
cTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGAc > 2:122355/1‑139 (MQ=255)
tGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACg < 1:222254/139‑1 (MQ=255)
taataGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTaa > 2:161478/1‑139 (MQ=255)
gCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGAt > 2:239384/1‑139 (MQ=255)
cATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATa > 2:511378/1‑139 (MQ=255)
tAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAata > 2:107869/1‑139 (MQ=255)
cAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATa > 2:87059/1‑139 (MQ=255)
|
ATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATA > NC_000913/4635260‑4635528
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
