Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	69	53.0	2252012	96.1%	2164183	115.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,430	G→A	P69L (CCG→CTG)	mepM ←	murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,430	0	G	A	100.0%	256.1 / NA	72	P69L (CCG→CTG)	mepM	murein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/35); total (37/35)

TACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAC  >  NC_000913/1941298‑1941564

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:50987/1‑139 (MQ=255)

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  2:846028/1‑139 (MQ=255)

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:572149/1‑139 (MQ=255)

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  2:728290/1‑139 (MQ=255)

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCAc                                                                                                                                  >  1:918062/1‑139 (MQ=255)

tACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATAAGCTCAc                                                                                                                                  >  1:925704/1‑139 (MQ=255)

 aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  2:308713/1‑139 (MQ=255)

 aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:136620/139‑1 (MQ=255)

 aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:423339/139‑1 (MQ=255)

 aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:935400/139‑1 (MQ=255)

 aCTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTCCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:231954/139‑1 (MQ=255)

  cTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTg                                                                                                                                >  1:557704/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  2:370699/139‑1 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:1033446/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:251631/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:452363/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:572589/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:618423/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:719386/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  2:730868/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:370699/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:1003006/1‑139 (MQ=255)

     aTTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  1:118893/139‑1 (MQ=255)

      ttGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCtt                                                                                                                            <  1:455104/139‑1 (MQ=255)

                   ttAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGcga                                                                                                               >  1:418795/1‑139 (MQ=255)

                         tATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAAt                                                                                                         >  2:402546/1‑139 (MQ=255)

                          aTCGCCAGTGGCAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAAtt                                                                                                        <  1:728290/139‑1 (MQ=255)

                            cGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  1:471727/112‑1 (MQ=255)

                            cGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  2:471727/1‑112 (MQ=255)

                               cAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGtt                                                                                                   <  1:693304/139‑1 (MQ=255)

                                 gTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCt                                                                                                 >  2:18608/1‑139 (MQ=255)

                                   ggAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGt                                                                                               <  2:1033446/139‑1 (MQ=255)

                                        caacaTATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAg                                                                                          <  2:276845/139‑1 (MQ=255)

                                              atTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAAt                                                                                    <  1:377907/139‑1 (MQ=255)

                                                    gCACGCCCGCTCCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGtttt                                                                              <  1:730868/139‑1 (MQ=255)

                                                         cccGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 <  2:149548/83‑1 (MQ=255)

                                                         cccGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACt                                                                                                                                 >  1:149548/1‑83 (MQ=255)

                                                           cGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAAtt                                                                       <  1:618423/139‑1 (MQ=255)

                                                             cTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTgg                                                                     >  1:565051/1‑139 (MQ=255)

                                                             cTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTgg                                                                     <  2:50987/139‑1 (MQ=255)

                                                               tCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATAAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCg                                                                   <  2:925704/139‑1 (MQ=255)

                                                                cACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGt                                                                  <  2:918062/139‑1 (MQ=255)

                                                                        aTTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCAc                                                          <  2:1106882/139‑1 (MQ=255)

                                                                                        cGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAgg                                          >  1:249087/1‑139 (MQ=255)

                                                                                          tCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             <  2:126231/54‑1 (MQ=255)

                                                                                          tCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCt                                                                                                                             >  1:126231/1‑54 (MQ=255)

                                                                                           ccTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTgg                                                                                                                               >  1:894738/1‑51 (MQ=255)

                                                                                           ccTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTgg                                                                                                                               <  2:894738/51‑1 (MQ=255)

                                                                                               gggAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAg                                   >  1:1123466/1‑139 (MQ=255)

                                                                                                    tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              >  1:343335/1‑139 (MQ=255)

                                                                                                    tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              <  1:308713/139‑1 (MQ=255)

                                                                                                    tGGCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGc                              >  1:710291/1‑139 (MQ=255)

                                                                                                     ggCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCg                             <  1:822797/139‑1 (MQ=255)

                                                                                                      gCTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGa                            <  2:719386/139‑1 (MQ=255)

                                                                                                       cTTCATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGAc                           >  2:938406/1‑139 (MQ=255)

                                                                                                          cATCTTCTTGTGCAGCTTTATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  1:846028/139‑1 (MQ=255)

                                                                                                          cATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  2:418795/139‑1 (MQ=255)

                                                                                                          cATCTTCTTGTGCAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCATGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGc                        <  1:643357/139‑1 (MQ=255)

                                                                                                                      cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATa                                             <  1:10722/106‑1 (MQ=255)

                                                                                                                      cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATa                                             >  2:10722/1‑106 (MQ=255)

                                                                                                                      cAGCTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAAc            >  1:74874/1‑139 (MQ=255)

                                                                                                                         cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCa                                                                                      >  2:365990/1‑62 (MQ=255)

                                                                                                                         cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCa                                                                                      <  1:365990/62‑1 (MQ=255)

                                                                                                                         cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGt         >  2:560119/1‑139 (MQ=255)

                                                                                                                         cTTGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGt         >  2:412132/1‑139 (MQ=255)

                                                                                                                          ttGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCa                                                           >  2:566208/1‑88 (MQ=255)

                                                                                                                          ttGATCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCa                                                           <  1:566208/88‑1 (MQ=255)

                                                                                                                             atcaatcaGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGc     <  1:251631/139‑1 (MQ=255)

                                                                                                                              tCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCaa                                                                                     >  2:638509/1‑58 (MQ=255)

                                                                                                                              tCAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCaa                                                                                     <  1:638509/58‑1 (MQ=255)

                                                                                                                               cAATCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGa   <  1:402546/139‑1 (MQ=255)

                                                                                                                                aaTCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAc  <  1:18608/139‑1 (MQ=255)

                                                                                                                                aaTCAGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGACCAGAGTAAGAACGGTGAGCGAc  <  2:572149/139‑1 (MQ=255)

TACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGAC  >  NC_000913/1941298‑1941564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A