Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
81 |
47.9 |
2225048 |
94.8% |
2109345 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,314,922 |
T→C |
S479P (TCC→CCC) |
rcsD → |
phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,314,922 | 0 | T | C | 100.0%
| 85.1
/ NA
| 24 | S479P (TCC→CCC) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/12); total (12/12) |
TTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATG > NC_000913/2314791‑2315039
|
ttCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGc > 1:104229/1‑139 (MQ=255)
aTCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGagag < 1:328387/139‑1 (MQ=255)
aaCAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCgg > 2:124264/1‑139 (MQ=255)
aaGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGaaa > 2:75719/1‑139 (MQ=255)
cgcAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGaa > 2:148501/1‑139 (MQ=255)
cAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATc < 2:1038919/139‑1 (MQ=255)
aGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCa < 1:124264/139‑1 (MQ=255)
gCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACTACTGGCGAATCAg < 1:959310/139‑1 (MQ=255)
gTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGc < 1:148501/139‑1 (MQ=255)
tCTGTATGAGAAAAACCAGCAGGGGCGGAAGATCTTTGTGAAATACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTATACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCa > 2:741510/1‑139 (MQ=255)
aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTgg > 2:857603/1‑139 (MQ=255)
aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg > 1:207219/1‑139 (MQ=255)
gggCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg < 2:104229/139‑1 (MQ=255)
gggCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg < 1:16739/139‑1 (MQ=255)
gatgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaa > 2:541677/1‑139 (MQ=255)
atgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa < 2:964261/139‑1 (MQ=255)
atCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATAc < 1:527392/139‑1 (MQ=255)
aTGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt < 1:162089/122‑1 (MQ=255)
aTGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt > 2:162089/1‑122 (MQ=255)
aaCATTGGAGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACTGTTAGCGAACa > 2:721691/1‑139 (MQ=255)
gcgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg > 1:450101/1‑139 (MQ=255)
gcgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg < 1:857603/139‑1 (MQ=255)
cgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa < 1:999912/111‑1 (MQ=255)
cgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa > 2:999912/1‑111 (MQ=255)
|
TTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATG > NC_000913/2314791‑2315039
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
