Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
81 |
47.9 |
2225048 |
94.8% |
2109345 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,380,852 |
T→C |
D59G (GAC→GGC) |
elaB ← |
putative membrane‑anchored DUF883 family ribosome‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,380,852 | 0 | T | C | 100.0%
| 95.4
/ NA
| 26 | D59G (GAC→GGC) | elaB | putative membrane‑anchored DUF883 family ribosome‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/15); total (11/15) |
CCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGC > NC_000913/2380733‑2380980
|
ccAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGTCTAACCCg < 2:722655/139‑1 (MQ=255)
aaCAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGttttt < 2:471543/139‑1 (MQ=255)
ccAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAg < 2:192627/139‑1 (MQ=255)
ccAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAg > 1:433000/1‑139 (MQ=255)
aGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAgcg > 1:311205/1‑139 (MQ=255)
aTTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGGTAAACTGCCTTCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCGAACCCGTTTTTTCACATCCTCCAGCGCTTTTTTTGCACGCGCTTTCAGCt < 1:562837/139‑1 (MQ=255)
aTTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGttttt < 2:486768/100‑1 (MQ=255)
aTTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGttttt > 1:486768/1‑100 (MQ=255)
ttCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTc < 2:1058688/139‑1 (MQ=255)
ttGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACa < 2:433000/139‑1 (MQ=255)
tGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat > 2:932676/1‑139 (MQ=255)
tGCCAGGGTTTTTCGTAGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat > 1:387277/1‑139 (MQ=255)
ggACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCggg < 2:881207/139‑1 (MQ=255)
aGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAgaggag < 1:343532/139‑1 (MQ=255)
tAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCa < 2:311205/139‑1 (MQ=255)
tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCggg < 1:486913/114‑1 (MQ=255)
tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCggg > 2:486913/1‑114 (MQ=255)
gcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAggagcggagc > 1:714744/1‑132 (MQ=255)
gcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAggagcggagc < 2:714744/132‑1 (MQ=255)
ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc > 1:468666/1‑139 (MQ=255)
gcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGc < 2:976999/114‑1 (MQ=255)
gcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGc > 1:976999/1‑114 (MQ=255)
gcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAg > 1:349634/1‑139 (MQ=255)
tAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt > 2:251072/1‑76 (MQ=255)
tAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt < 1:251072/76‑1 (MQ=255)
gTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAagc < 1:304105/139‑1 (MQ=255)
|
CCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTGTTTCACTAAGC > NC_000913/2380733‑2380980
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
