Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,380,852	T→C	D59G (GAC→GGC)	elaB ←	putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,852	0	T	C	100.0%	122.4 / NA	33	D59G (GAC→GGC)	elaB	putative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/14); total (19/14)

TTTAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTG  >  NC_000913/2380721‑2380969

tttAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGc                                                                                                                <  2:90665/139‑1 (MQ=255)

        cGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTaa                                                                                                        >  1:396253/1‑139 (MQ=255)

         gTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAAc                                                                                                       >  2:777862/1‑139 (MQ=255)

                      tCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTcaca                                                                                          >  1:604931/1‑139 (MQ=255)

                              ccAGCCCAACGGCTGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAg                                                                                  <  1:209864/139‑1 (MQ=255)

                                aGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTCTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAgcg                                                                                >  2:296834/1‑139 (MQ=255)

                                  cccAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCt                                                                              >  2:34969/1‑139 (MQ=255)

                                      aCGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCttttt                                                                          >  1:300134/1‑139 (MQ=255)

                                             cgcCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCAcg                                                                   <  2:396253/139‑1 (MQ=255)

                                                        aTTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        >  2:122167/1‑139 (MQ=255)

                                                            cTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAAc                                                    <  1:777862/139‑1 (MQ=255)

                                                              tGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat                                                  >  1:661269/1‑139 (MQ=255)

                                                              tGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat                                                  >  1:726542/1‑139 (MQ=255)

                                                                ccAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACatat                                                >  1:662130/1‑139 (MQ=255)

                                                                            gTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggcg                                    <  2:300134/139‑1 (MQ=255)

                                                                                  gTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCg                              >  2:185414/1‑139 (MQ=255)

                                                                                          caGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATAGTTCTGATCGGCGGGATCGCCAgagga                      <  2:604931/139‑1 (MQ=255)

                                                                                           aGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAgaggag                     <  2:320501/139‑1 (MQ=255)

                                                                                             cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCg                                                                                                    <  1:206322/58‑1 (MQ=255)

                                                                                             cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCg                                                                                                    >  2:206322/1‑58 (MQ=255)

                                                                                             cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggc                                     <  2:29949/121‑1 (MQ=255)

                                                                                             cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggc                                     >  1:29949/1‑121 (MQ=255)

                                                                                                gATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAggagcggag                >  1:450604/1‑139 (MQ=255)

                                                                                                       tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACctct         >  1:753713/1‑139 (MQ=255)

                                                                                                       tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACctct         >  1:150373/1‑139 (MQ=255)

                                                                                                         cctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTc       <  1:88847/139‑1 (MQ=255)

                                                                                                         cctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTc       <  2:661269/139‑1 (MQ=255)

                                                                                                          ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        >  2:274550/1‑89 (MQ=255)

                                                                                                          ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        <  1:274550/89‑1 (MQ=255)

                                                                                                          ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      >  1:41932/1‑139 (MQ=255)

                                                                                                              ttCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAgtg  <  2:41932/139‑1 (MQ=255)

                                                                                                                  cccGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAGCATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      >  2:426695/1‑131 (MQ=255)

                                                                                                                  cccGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      <  1:426695/131‑1 (MQ=255)

TTTAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTG  >  NC_000913/2380721‑2380969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A