Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,834,077	G→A	A56T (GCC→ACC)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,834,077	0	G	A	100.0%	139.0 / NA	41	A56T (GCC→ACC)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/17); total (24/17)

TTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAA  >  NC_000913/2833973‑2834208

ttCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAAtt                                                                                                   >  1:1002407/1‑139 (MQ=255)

      aaCAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa                                                                                             <  1:358363/139‑1 (MQ=255)

                   tATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTg                                                                                <  2:218659/139‑1 (MQ=255)

                     ttCCATTAACCCCGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGc                                                                                                                    <  1:1110951/101‑1 (MQ=255)

                     ttCCATTAACCCCGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGc                                                                                                                    >  2:1110951/1‑101 (MQ=255)

                          ttAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  2:878079/1‑139 (MQ=255)

                          ttAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  1:303729/1‑139 (MQ=255)

                             aCCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTc                                                                      >  2:726888/1‑139 (MQ=255)

                              cccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTcc                                                                     <  1:214956/139‑1 (MQ=255)

                               ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:176252/1‑139 (MQ=255)

                               ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:19714/1‑139 (MQ=255)

                               ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGAAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:1061549/1‑139 (MQ=255)

                                  gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                                                                                            <  2:634944/112‑1 (MQ=255)

                                  gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                                                                                            >  1:634944/1‑112 (MQ=255)

                                           cgACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTg                                                        <  1:878079/139‑1 (MQ=255)

                                            gACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGa                                                       <  2:206764/139‑1 (MQ=255)

                                             aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGcc                                                                                               >  1:1102214/1‑98 (MQ=255)

                                             aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCa                                                                              <  2:365736/115‑1 (MQ=255)

                                             aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCa                                                                              >  1:365736/1‑115 (MQ=255)

                                             aCAGCATGGATGAGTAAAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGcc                                                                                               <  2:1102214/98‑1 (MQ=255)

                                                     gATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCg                                              <  2:1002407/139‑1 (MQ=255)

                                                         aGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGc                                          >  1:264869/1‑139 (MQ=255)

                                                           tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         <  2:438207/106‑1 (MQ=255)

                                                           tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  1:438207/1‑106 (MQ=255)

                                                           tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCg                                        >  1:681865/1‑139 (MQ=255)

                                                              aacaaACCTGATCTCAGCCAAGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAg                                     >  1:898693/1‑139 (MQ=255)

                                                                caaACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGcc                                   >  1:1082118/1‑139 (MQ=255)

                                                                     cTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCgtgt                              >  1:521675/1‑139 (MQ=255)

                                                                        aCCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTgtggt                           >  1:899244/1‑139 (MQ=255)

                                                                          cTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGa                         >  2:432715/1‑139 (MQ=255)

                                                                               ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGc                    <  2:959739/139‑1 (MQ=255)

                                                                               ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGc                    <  2:521675/139‑1 (MQ=255)

                                                                                cATGTTATCAGTCAGGGTCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTTGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcg                   <  2:681865/139‑2 (MQ=255)

                                                                                             agGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTg      >  1:499524/1‑139 (MQ=255)

                                                                                              ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcc                   <  1:914418/125‑1 (MQ=255)

                                                                                              ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcc                   >  2:914418/1‑125 (MQ=255)

                                                                                              ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTgg     >  1:270637/1‑139 (MQ=255)

                                                                                              ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTgg     >  1:820751/1‑139 (MQ=255)

                                                                                               gggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGc    >  1:624117/1‑139 (MQ=255)

                                                                                                 gCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCaa  <  2:499524/139‑1 (MQ=255)

                                                                                                 gCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCaa  <  2:270637/139‑1 (MQ=255)

TTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAA  >  NC_000913/2833973‑2834208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A