Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,121	A→G	N269S (AAC→AGC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,121	0	A	G	100.0%	169.2 / NA	45	N269S (AAC→AGC)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/16); total (29/16)

TCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCA  >  NC_000913/3931985‑3932256

tCGATTACGGGGGACGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGcc                                                                                                                                       >  2:595026/1‑139 (MQ=255)

     tACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGaa                                                                                                                                  >  2:688463/1‑139 (MQ=255)

                  gcgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAAcc                                                                                                                     <  2:51839/139‑1 (MQ=255)

                                  tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                                     >  1:111110/1‑139 (MQ=255)

                                  tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                                     >  2:544435/1‑139 (MQ=255)

                                    gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCgg                                                                                                                                    <  2:995271/106‑1 (MQ=255)

                                    gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCgg                                                                                                                                    >  1:995271/1‑106 (MQ=255)

                                    gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAc                                                                                                   >  2:838007/1‑139 (MQ=255)

                                    gggCACCTTGGTAACTTCCCTATTCACCTGGCGTGGTTCACCGTCGCATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAc                                                                                                   >  1:1029184/1‑139 (MQ=255)

                                                 aGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCt                                                                                      <  1:544435/139‑1 (MQ=255)

                                                      ccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                                 >  2:39376/1‑139 (MQ=255)

                                                      ccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                                 <  2:371354/139‑1 (MQ=255)

                                                        tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGa                                                                                                                        >  1:177152/1‑98 (MQ=255)

                                                        tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGa                                                                                                                        <  2:177152/98‑1 (MQ=255)

                                                        tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCg                                                                               >  2:1060248/1‑139 (MQ=255)

                                                                       gTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgcc                                                                >  2:394917/1‑139 (MQ=255)

                                                                        ttCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgccg                                                               >  1:469984/1‑139 (MQ=255)

                                                                            ccGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACt                                                           >  1:390784/1‑139 (MQ=255)

                                                                               tcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                           <  1:710774/88‑1 (MQ=255)

                                                                               tcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                           >  2:710774/1‑88 (MQ=255)

                                                                                cgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCg                                                       >  2:163019/1‑139 (MQ=255)

                                                                                  tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGa                                                                                               >  1:445441/1‑97 (MQ=255)

                                                                                  tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGa                                                                                               <  2:445441/97‑1 (MQ=255)

                                                                                  tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGAc                                                     >  1:424749/1‑139 (MQ=255)

                                                                                           ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGc                                                                                                                                <  2:841664/55‑1 (MQ=255)

                                                                                           ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGc                                                                                                                                >  1:841664/1‑55 (MQ=255)

                                                                                           ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGc                                            >  1:1077898/1‑139 (MQ=255)

                                                                                                 cccTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCa                                      <  1:595026/139‑1 (MQ=255)

                                                                                                       aTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                                <  1:688463/139‑1 (MQ=255)

                                                                                                       aTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                                <  1:838007/139‑1 (MQ=255)

                                                                                                            ttCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTAtct                           >  1:277699/1‑139 (MQ=255)

                                                                                                             tCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTAtctc                          >  2:441391/1‑139 (MQ=255)

                                                                                                              cGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACCGGCGACGGTAATTGCCTCGCAGGCGGTTATctct                         >  2:399181/1‑139 (MQ=255)

                                                                                                               ggTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTg                        >  2:642379/1‑139 (MQ=255)

                                                                                                                gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCg                                                                                                                   <  2:13674/47‑1 (MQ=255)

                                                                                                                gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCg                                                                                                                   >  1:13674/1‑47 (MQ=255)

                                                                                                                gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                       >  1:318556/1‑139 (MQ=255)

                                                                                                                gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                       >  2:707468/1‑139 (MQ=255)

                                                                                                                 tCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                      <  1:395490/139‑1 (MQ=255)

                                                                                                                 tCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                      <  1:83448/139‑1 (MQ=255)

                                                                                                                    gggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtc                   <  2:1077898/139‑1 (MQ=255)

                                                                                                                     ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtct                  >  1:744386/1‑139 (MQ=255)

                                                                                                                     ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtct                  >  2:390478/1‑139 (MQ=255)

                                                                                                                         cgcTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTtctc              <  1:390478/139‑1 (MQ=255)

                                                                                                                                   aaaGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGt    >  1:284432/1‑139 (MQ=255)

                                                                                                                                     agagCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCa  <  2:113242/139‑1 (MQ=255)

TCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCA  >  NC_000913/3931985‑3932256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A