Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 78 65.3 3016648 94.6% 2853749 111.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,274,204 G→A A235V (GCA→GTA)  garP ← putative (D)‑galactarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,274,2040GA100.0% 234.5 / NA 66A235V (GCA→GTA) garPputative (D)‑galactarate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/36);  total (30/36)

TGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGC  >  NC_000913/3274072‑3274329
                                                                                                                                    |                                                                                                                             
tGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCg                                                                                                                         <  2:813405/139‑1 (MQ=255)
       gATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGcc                                                                                                                  >  2:1106846/1‑139 (MQ=255)
       gATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGcc                                                                                                                  <  1:398898/139‑1 (MQ=255)
                ggAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  2:1127577/139‑1 (MQ=255)
                ggAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:856722/1‑139 (MQ=255)
                 gAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGc                                                                                                              >  2:165515/1‑133 (MQ=255)
                 gAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGc                                                                                                              <  1:165515/133‑1 (MQ=255)
                       ggTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTcc                                                                                                  <  2:73463/139‑1 (MQ=255)
                       ggTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTcc                                                                                                  <  1:319794/139‑1 (MQ=255)
                            ggAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTtgtg                                                                                                      <  1:440709/130‑1 (MQ=255)
                            ggAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTtgtg                                                                                                      >  2:440709/1‑130 (MQ=255)
                                  aCCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGaccac                                                                                       >  2:505301/1‑139 (MQ=255)
                                   ccAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGaccacc                                                                                      >  1:1268318/1‑139 (MQ=255)
                                             gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:856722/139‑1 (MQ=255)
                                             gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:126238/139‑1 (MQ=255)
                                             gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  2:97261/139‑1 (MQ=255)
                                                 tGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCaga                                                                        >  2:762135/1‑139 (MQ=255)
                                                       aaTATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            <  1:205363/129‑1 (MQ=255)
                                                       aaTATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCAtttt                                                                            >  2:205363/1‑129 (MQ=255)
                                                          atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCag                                                                         >  1:1382126/1‑129 (MQ=255)
                                                          atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCag                                                                         <  2:1382126/129‑1 (MQ=255)
                                                            tGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCa                                                             >  1:1018631/1‑139 (MQ=255)
                                                             gTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  2:180280/1‑139 (MQ=255)
                                                             gTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCATGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  2:1064452/1‑139 (MQ=255)
                                                              tCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGGTAAACTTCAGc                                                           >  1:978858/1‑139 (MQ=255)
                                                               ccGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGct                                                          <  2:1295651/139‑1 (MQ=255)
                                                                    aaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:482702/139‑1 (MQ=255)
                                                                    aaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:978858/139‑1 (MQ=255)
                                                                     aaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    <  1:762135/139‑1 (MQ=255)
                                                                        aCGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAg                                                 <  1:1106846/139‑1 (MQ=255)
                                                                             cAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            <  2:788574/139‑1 (MQ=255)
                                                                             cAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            >  1:1267813/1‑139 (MQ=255)
                                                                                catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            <  2:543773/136‑1 (MQ=255)
                                                                                catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                            >  1:543773/1‑136 (MQ=255)
                                                                                catcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGt                                         <  1:1288126/139‑1 (MQ=255)
                                                                                     tGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCat                                                                                                >  1:1114655/1‑79 (MQ=255)
                                                                                     tGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCat                                                                                                <  2:1114655/79‑1 (MQ=255)
                                                                                       cGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGAt                                  <  2:1268318/139‑1 (MQ=255)
                                                                                            agagagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTc                             >  1:1075834/1‑139 (MQ=255)
                                                                                                 gCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGtt                        <  2:1018631/139‑1 (MQ=255)
                                                                                                 gCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGtt                        <  1:799103/139‑1 (MQ=255)
                                                                                                  cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTc                                                                           <  2:137134/87‑1 (MQ=255)
                                                                                                  cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTc                                                                           >  1:137134/1‑87 (MQ=255)
                                                                                                  cAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTa                       >  2:1239691/1‑139 (MQ=255)
                                                                                                    aTTGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATg                     >  2:692551/1‑139 (MQ=255)
                                                                                                      tGCTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGaa                   <  2:1267813/139‑1 (MQ=255)
                                                                                                        cTTGATGTAATGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATc                 >  1:591021/1‑139 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:285456/1‑41 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  1:1496811/41‑1 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         <  1:285456/41‑1 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCttttt                                                                                                         >  2:1496811/1‑41 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACcgc                                                                                    <  2:438271/62‑1 (MQ=255)
                                                                                                                  tGCAGTTTGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACcgc                                                                                    >  1:438271/1‑62 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGt                                                                                                    <  1:1329305/41‑1 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGt                                                                                                    >  2:1329305/1‑41 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    <  2:295727/89‑1 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCg                                                    >  1:295727/1‑89 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAAt                  <  2:382924/123‑1 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAAt                  >  1:382924/1‑123 (MQ=255)
                                                                                                                       tttGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAgc  <  1:692551/139‑1 (MQ=255)
                                                                                                                         tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            <  2:730165/79‑1 (MQ=255)
                                                                                                                         tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                            >  1:730165/1‑79 (MQ=255)
                                                                                                                         tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGt                                     >  2:430962/1‑102 (MQ=255)
                                                                                                                         tGGGTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGt                                     <  1:430962/102‑1 (MQ=255)
                                                                                                                            gTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     <  2:771834/83‑1 (MQ=255)
                                                                                                                            gTCCGCTTACTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                     >  1:771834/1‑83 (MQ=255)
                                                                                                                                    |                                                                                                                             
TGCACCAGATAAATCGGGAACCAGGTGAGGAAGAACCAGGTGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGC  >  NC_000913/3274072‑3274329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A