Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 65 21.2 1002576 96.5% 967485 111.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 585,420 Δ1 bp coding (214/954 nt) ompT ← DLP12 prophage, outer membrane protease VII, outer membrane protein 3b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913585,4200T.100.0% 38.6 / NA 10coding (214/954 nt)ompTDLP12 prophage, outer membrane protease VII, outer membrane protein 3b
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/6);  total (4/6)

TCCAGGTTCCGGGGTTACTGGAATCCATCCAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGT  >  NC_000913/585288‑585552
                                                                                                                                    |                                                                                                                                    
tCCAGGTTCCGGGGTTACTGGAATCCATCCAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAA‑AATTGCa                                                                                                                               <  1:444669/139‑1 (MQ=255)
                                                   cACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATACCTGGGGCATCAAATCCCGATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCCTCg                                                                            <  1:322905/139‑1 (MQ=255)
                                                                                                    gggCATCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCgg                                                                                        <  1:480319/78‑1 (MQ=255)
                                                                                                    gggCATCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCgg                                                                                        >  2:480319/1‑78 (MQ=255)
                                                                                                        aTCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTc                       <  2:168789/139‑1 (MQ=255)
                                                                                                         tCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCaga                                        >  1:191490/1‑121 (MQ=255)
                                                                                                         tCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCaga                                        <  2:191490/121‑1 (MQ=255)
                                                                                                         tCAAATCCCAATTAATTGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTcc                      >  2:471260/1‑139 (MQ=255)
                                                                                                                    ttaattGCACCTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTAtgtt           >  2:438953/1‑139 (MQ=255)
                                                                                                                             ccTTTAA‑AATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGt  <  1:438953/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TCCAGGTTCCGGGGTTACTGGAATCCATCCAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCCAGTCGAGTTGACTGACTTTTCGGCCTCCTTCTTCGGCTAGATAAACACGCTCTTTTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGT  >  NC_000913/585288‑585552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A