Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	65	21.2	1002576	96.5%	967485	111.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,941	G→A	A357V (GCC→GTC)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,941	0	G	A	100.0%	101.5 / NA	30	A357V (GCC→GTC)	yhaO	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/19); total (11/19)

CTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAC  >  NC_000913/3256807‑3257065

cTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGCTTTTCGTTAATCTTCTCgacagg                                                                                                                          >  1:76412/1‑139 (MQ=255)

         cGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCg                                                                                                                 >  1:394435/1‑139 (MQ=255)

           aaaaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCTCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGAc                                                                                                               <  2:394435/139‑1 (MQ=255)

           aaaaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGAc                                                                                                               >  2:359509/1‑139 (MQ=255)

             aaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGc                                                                                                             <  2:206824/139‑1 (MQ=255)

                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  2:83643/139‑1 (MQ=255)

                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  1:317430/139‑1 (MQ=255)

                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  2:487869/139‑1 (MQ=255)

                 gggCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGa                                                                                                         <  2:415532/139‑1 (MQ=255)

                                 tGAAGCTCAACCCCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATccc                                                                                         >  1:27756/1‑139 (MQ=255)

                                   aaGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCtt                                                                                       >  1:102745/1‑139 (MQ=255)

                                      cTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCg                                                                                    <  1:104752/139‑1 (MQ=255)

                                       tCAACCCCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGt                                                                                   <  2:27756/139‑1 (MQ=255)

                                         aaCACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTg                                                                                 >  2:425742/1‑139 (MQ=255)

                                                gTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTcgcg                                                                          <  2:431089/139‑1 (MQ=255)

                                                gTGAGTTCAGTACGATGGCGCTCCATGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTcgcg                                                                          <  2:323781/139‑1 (MQ=255)

                                                     ttCAGTACCATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGATTCGCGAAAGc                                                                     >  2:326384/1‑139 (MQ=255)

                                                              aTGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGa                                                            <  1:345496/139‑1 (MQ=255)

                                                               tGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCa                                                                                                   >  2:345510/1‑99 (MQ=255)

                                                               tGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCa                                                                                                   <  1:345510/99‑1 (MQ=255)

                                                                  cgcTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                               <  2:60516/100‑1 (MQ=255)

                                                                  cgcTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                               >  1:60516/1‑100 (MQ=255)

                                                                               cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGtt                                                                                                     >  1:362968/1‑81 (MQ=255)

                                                                               cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGtt                                                                                                     <  2:362968/81‑1 (MQ=255)

                                                                                        gAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCa                                  <  1:359509/139‑1 (MQ=255)

                                                                                             tCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGat                             <  1:380905/139‑1 (MQ=255)

                                                                                                tgatgCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATAtt                          <  2:102745/139‑1 (MQ=255)

                                                                                                             aaCGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTg             >  2:419484/1‑139 (MQ=255)

                                                                                                                       tCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGAt                                                                                                        >  1:179772/1‑38 (MQ=255)

                                                                                                                       tCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGAt                                                                                                        <  2:179772/38‑1 (MQ=255)

                                                                                                                        cGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAc  <  1:419484/139‑1 (MQ=255)

CTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAC  >  NC_000913/3256807‑3257065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A