Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
65 |
21.2 |
1002576 |
96.5% |
967485 |
111.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,160,278 |
G→A |
S171S (AGC→AGT) |
sthA ← |
pyridine nucleotide transhydrogenase, soluble |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,160,278 | 0 | G | A | 100.0%
| 92.0
/ NA
| 27 | S171S (AGC→AGT) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/14); total (13/14) |
CCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCC > NC_000913/4160184‑4160397
|
ccACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATc > 1:253061/1‑139 (MQ=255)
cATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga < 2:128384/139‑1 (MQ=255)
aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGaa > 2:41601/1‑122 (MQ=255)
aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGaa < 1:41601/122‑1 (MQ=255)
aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCaa < 1:165258/127‑1 (MQ=255)
aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCaa > 2:165258/1‑127 (MQ=255)
aCCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatga > 2:88945/1‑139 (MQ=255)
cgcgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGata > 2:261853/1‑139 (MQ=255)
cgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATg > 1:50822/1‑139 (MQ=255)
cgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATg > 1:202890/1‑139 (MQ=255)
gAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGa < 2:50822/139‑1 (MQ=255)
aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAg > 1:425133/1‑60 (MQ=255)
aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAg < 2:425133/60‑1 (MQ=255)
tCCAGCACCATATATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAGCTGTTGGATGAGATGGACGAGAGCCGCAGGCAATAACAAATTTTTCa < 1:261853/139‑1 (MQ=255)
cACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGt < 1:88945/139‑1 (MQ=255)
cACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGt < 2:202890/139‑1 (MQ=255)
cATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAg < 2:333902/139‑1 (MQ=255)
tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAt > 1:384772/1‑82 (MQ=255)
tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAt < 2:384772/82‑1 (MQ=255)
tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGc > 1:182540/1‑109 (MQ=255)
tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGc < 2:182540/109‑1 (MQ=255)
aGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAgtgt < 1:88167/139‑1 (MQ=255)
cATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTc > 2:218326/1‑43 (MQ=255)
cATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTc < 1:218326/43‑1 (MQ=255)
ggCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGcc > 2:166027/1‑139 (MQ=255)
cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACga < 2:198489/87‑1 (MQ=255)
cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACga > 1:198489/1‑87 (MQ=255)
|
CCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCC > NC_000913/4160184‑4160397
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
