Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	65	21.2	1002576	96.5%	967485	111.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,415,887	(G)_6→7	coding (1613/1626 nt)	aidB →	DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,415,881	1	.	G	100.0%	99.6 / NA	28	T536R (ACG→AGG)	aidB	DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (15/13); total (15/13)

TGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑GGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGA  >  NC_000913/4415746‑4416013

tgctgGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑ggg                                                                                                                                   >  1:376346/1‑139 (MQ=255)

 gctgGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑gggg                                                                                                                                  <  2:286226/139‑1 (MQ=255)

            gTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtg                                                                                                                        >  1:334324/1‑139 (MQ=255)

              gcgcAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                      <  2:386399/139‑1 (MQ=255)

                  aaaTGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAg                                                                                                                  <  2:996/139‑1 (MQ=255)

                               tGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑gggggggagtg                                                                                                                           <  1:202870/116‑6 (MQ=255)

                               tGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑gggggggagtg                                                                                                                           >  2:202870/1‑111 (MQ=255)

                                 cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAc                                                                                                                                       <  1:365791/103‑1 (MQ=255)

                                 cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAc                                                                                                                                       >  2:365791/1‑103 (MQ=255)

                                    ctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGa                                                                                                >  1:215816/1‑139 (MQ=255)

                                    ctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGa                                                                                                >  2:378365/1‑139 (MQ=255)

                                                       gtggtgTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTa                                                                             <  2:376346/139‑1 (MQ=255)

                                                               aGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAat                                                                     >  2:378497/1‑139 (MQ=255)

                                                                    aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGaaa                                                                      <  2:47246/133‑1 (MQ=255)

                                                                    aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATAAGACTGATGCGACGCTGGTTTCGATTAACTAAATGaaa                                                                      >  1:47246/1‑133 (MQ=255)

                                                                     tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                >  1:359495/1‑122 (MQ=255)

                                                                     tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                <  2:359495/122‑1 (MQ=255)

                                                                              cgcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAg                                                      >  1:96149/1‑139 (MQ=255)

                                                                                                  tCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCgcgc                                  >  1:164954/1‑139 (MQ=255)

                                                                                                   cAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGcgcc                                 <  2:215816/139‑1 (MQ=255)

                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                <  2:195435/84‑1 (MQ=255)

                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                >  1:195435/1‑84 (MQ=255)

                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCgg                         >  1:453911/1‑139 (MQ=255)

                                                                                                                  aTGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGtt                  >  1:237745/1‑139 (MQ=255)

                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGa                                                                                    <  1:91895/70‑1 (MQ=255)

                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGa                                                                                    >  2:91895/1‑70 (MQ=255)

                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               <  2:334324/139‑1 (MQ=255)

                                                                                                                           tgctgcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               >  1:466344/1‑133 (MQ=255)

                                                                                                                           tgctgcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               <  2:466344/133‑1 (MQ=255)

                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                                  <  1:387661/94‑1 (MQ=255)

                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                                  >  2:387661/1‑94 (MQ=255)

                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTGTCGATTAACTAAATGAAATATGGGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGa     >  1:184922/1‑139 (MQ=255)

                                                                                                                                  ggCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGa  <  1:378497/139‑1 (MQ=255)

                                                                                                                                  ggCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGa  <  1:378365/139‑1 (MQ=255)

TGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑GGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGA  >  NC_000913/4415746‑4416013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A