Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
65 |
21.2 |
1002576 |
96.5% |
967485 |
111.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,595,843 |
A→G |
R3R (CGT→CGC) |
lgoR ← |
putative transcriptional activator for L‑galactonate catabolism |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,595,843 | 0 | A | G | 100.0%
| 54.3
/ NA
| 15 | R3R (CGT→CGC) | lgoR | putative transcriptional activator for L‑galactonate catabolism |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/7); total (8/7) |
ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCA > NC_000913/4595711‑4595967
|
atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAAGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc < 2:49156/139‑1 (MQ=255)
gtACATTTCCGCCAGCGCACTTCGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATAttt < 1:490788/118‑1 (MQ=255)
gtACATTTCCGCCAGCGCACTTCGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATAttt > 2:490788/1‑118 (MQ=255)
ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGACGGGATATGACCCCGTGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt < 2:318500/122‑1 (MQ=255)
ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGTGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt > 1:318500/1‑122 (MQ=255)
tGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGt < 1:245585/110‑1 (MQ=255)
tGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGt > 2:245585/1‑110 (MQ=255)
aGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc < 1:422395/73‑1 (MQ=255)
aGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc > 2:422395/1‑73 (MQ=255)
cgcgCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTttccg > 1:429343/1‑139 (MQ=255)
ttGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt < 1:416467/43‑1 (MQ=255)
ttGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt > 2:416467/1‑43 (MQ=255)
tgtgGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTaaa < 1:39603/66‑1 (MQ=255)
tgtgGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTaaa > 2:39603/1‑66 (MQ=255)
cGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGca > 1:1414/1‑139 (MQ=255)
|
ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCA > NC_000913/4595711‑4595967
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
