Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
71 |
51.6 |
2219666 |
95.9% |
2128659 |
118.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,001,154 |
T→C |
C270C (TGT→TGC) |
xdhA → |
xanthine dehydrogenase, molybdenum binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,001,154 | 0 | T | C | 97.0%
| 109.2
/ NA
| 33 | C270C (TGT→TGC) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (16/16); total (16/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGC > NC_000913/3001022‑3001270
|
aCGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTc < 2:1022483/139‑1 (MQ=255)
cGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg < 2:945117/139‑1 (MQ=255)
aTTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGc > 1:65774/1‑139 (MQ=255)
tGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCACCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCAc < 1:18511/139‑1 (MQ=255)
tACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGaa > 1:145305/1‑139 (MQ=255)
aCTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAAc > 2:861451/1‑139 (MQ=255)
aCTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCATGAAc > 1:569694/1‑139 (MQ=255)
ggAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc > 1:401944/1‑139 (MQ=255)
gagCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACg > 1:427380/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg < 2:849449/80‑1 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCg > 1:849449/1‑80 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAc < 1:343214/136‑1 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGAc > 2:343214/1‑136 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa > 1:974958/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa > 2:798858/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa > 2:1022631/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa > 1:472015/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa > 2:962636/1‑139 (MQ=255)
gCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGa < 2:974958/139‑1 (MQ=255)
ccAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa > 1:994117/1‑139 (MQ=255)
ccAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGaa < 2:65774/139‑1 (MQ=255)
cggcATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaa < 2:638062/139‑1 (MQ=255)
cggcATCCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTAAAGAGTGTTTCCTCGCACCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGaa < 1:384881/139‑1 (MQ=255)
ttCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGtt < 2:16431/139‑1 (MQ=255)
tCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTta < 1:268252/139‑1 (MQ=255)
tCCTGACCAGCAAGCTTGGCGACATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTta < 2:653534/139‑1 (MQ=255)
cAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGAt > 2:576040/1‑139 (MQ=255)
gCTTGGCGGCATTCCGTTGAAAGTTTCCCTTAGCCGGGAAGAGTGCTTCCTCGCAACCCGAACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGtt < 2:458371/139‑1 (MQ=255)
cGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCgg < 2:411215/139‑1 (MQ=255)
tGAAAGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGgcgc < 2:427380/139‑1 (MQ=255)
aGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt < 2:472015/139‑1 (MQ=255)
aGTTTCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGAAGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTAt > 1:41637/1‑139 (MQ=255)
tttCCCTTAGCCGTGAAGAGTGCTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGc < 2:994117/139‑1 (MQ=255)
|
ACGAGTCATCAAACCATTTGTCGGTGGCGGTTTTGGTAATAAACAGGATGTACTGGAAGAGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCCTTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGC > NC_000913/3001022‑3001270
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
