Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	71	51.6	2219666	95.9%	2128659	118.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,005,344	A→G	L148P (CTT→CCT)	ygeV ←	putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,005,344	0	A	G	100.0%	204.8 / NA	55	L148P (CTT→CCT)	ygeV	putative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/22); total (33/22)

TTTTTAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAAGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTAAA  >  NC_000913/3005213‑3005475

ttttgAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTCACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGa                                                                                                                              <  2:346332/139‑1 (MQ=255)

  tttAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATTATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGaaa                                                                                                                            >  2:685344/1‑139 (MQ=255)

           gTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   <  1:1109395/139‑1 (MQ=255)

            tCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtggt                                                                                                                  <  1:57890/139‑1 (MQ=255)

            tCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtggt                                                                                                                  >  2:78820/1‑139 (MQ=255)

                    aCAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGatatat                                                                                                          >  2:690389/1‑139 (MQ=255)

                      aaaCTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATgg                                                                                                        <  1:515217/139‑1 (MQ=255)

                          tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGttt                                                                                                                                 <  2:547780/110‑1 (MQ=255)

                          tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGttt                                                                                                                                 >  1:547780/1‑110 (MQ=255)

                          tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   >  2:1000913/1‑124 (MQ=255)

                          tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAAtgg                                                                                                                   <  1:1000913/124‑1 (MQ=255)

                          tGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGaa                                                                                                    >  1:153204/1‑139 (MQ=255)

                            aaCCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGCTCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  <  2:56116/139‑1 (MQ=255)

                              cccGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATa                                                                                                >  1:536520/1‑139 (MQ=255)

                                             cTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 <  1:690389/139‑1 (MQ=255)

                                             cTACAACTAATACGCCCTCATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 >  1:618288/1‑139 (MQ=255)

                                                  aCTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAAt                                                                            >  1:880442/1‑139 (MQ=255)

                                                     aaTACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAGCAAAAATGGAGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTAt                                                                         <  1:685344/139‑1 (MQ=255)

                                                      aTACGCCCTGATCCATATTATCGACCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCTTAAATTATc                                                                        >  1:750446/1‑139 (MQ=255)

                                                       tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       <  2:536520/139‑1 (MQ=255)

                                                       tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       >  1:246717/1‑139 (MQ=255)

                                                       tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       <  2:760179/139‑1 (MQ=255)

                                                       tACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCa                                                                       >  2:168830/1‑139 (MQ=255)

                                                            ccTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGGTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGat                                                                  >  1:740581/1‑139 (MQ=255)

                                                             cTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGata                                                                 <  2:350419/139‑1 (MQ=255)

                                                              tGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGatat                                                                >  2:307891/1‑139 (MQ=255)

                                                               gATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATg                                                               <  2:939562/139‑1 (MQ=255)

                                                               gATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATg                                                               <  2:695140/139‑1 (MQ=255)

                                                                aTCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGc                                                              >  2:128372/1‑139 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  >  2:603502/1‑93 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACa                                                                                                  <  1:603502/93‑1 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCt                                                    >  2:1067231/1‑139 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCt                                                    >  1:980969/1‑139 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTAGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 >  2:80186/1‑110 (MQ=255)

                                                                          tCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTAGATATATGGCGAACATAATCAGAAAATTcgcg                                                                                 <  1:80186/110‑1 (MQ=255)

                                                                               cATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTgg                                               >  2:498384/1‑139 (MQ=255)

                                                                               cATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTgg                                               >  1:966528/1‑139 (MQ=255)

                                                                                 tGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGt                                             <  2:510179/139‑1 (MQ=255)

                                                                                       cAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGc                                       >  2:911006/1‑139 (MQ=255)

                                                                                              tttACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCaaa                                >  2:225492/1‑139 (MQ=255)

                                                                                                tACTGATGTTATCTCCTGGCCCCTGATCCGCCAGAGGTTTCGAAAGAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACt                              >  1:641941/1‑139 (MQ=255)

                                                                                                    gATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATc                          >  1:24815/1‑139 (MQ=255)

                                                                                                    gATGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATc                          >  2:415236/1‑139 (MQ=255)

                                                                                                     aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTAt                           <  2:341630/137‑1 (MQ=255)

                                                                                                     aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTAt                           >  1:341630/1‑137 (MQ=255)

                                                                                                     aTGTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCa                         >  1:290079/1‑139 (MQ=255)

                                                                                                       gTTATCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCAcc                       >  1:298868/1‑139 (MQ=255)

                                                                                                          aTCTCCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCa                    <  1:415236/139‑1 (MQ=255)

                                                                                                           tctcCTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCaa                   <  1:911006/139‑1 (MQ=255)

                                                                                                               cTGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAAcaca               >  2:90965/1‑139 (MQ=255)

                                                                                                                tGGCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACaa              <  2:366400/139‑1 (MQ=255)

                                                                                                                 ggCCCCTGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCAAACACAAc             >  1:1028497/1‑139 (MQ=255)

                                                                                                                       tGATCCTCCAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCt       >  2:1105890/1‑139 (MQ=255)

                                                                                                                           cctccAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTaa   <  1:307891/139‑1 (MQ=255)

                                                                                                                            ctccAGAGGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTaaa  >  2:445144/1‑139 (MQ=255)

TTTTTAAGGCAGTCTGATTAACAAACTGAACCCGATTTTCATCATCTACAACTAATACGCCCTGATCCATATTATCGATCATGGTCGCAAATATTTTACTGATGTTATCTCCTGGCCCCTGATCCTCCAGAAGTTTCGAAACAAAAATGGTGGATATATGGCGAACATAATCAGAAAATTCGCGTAAATTATCACTGATATGCTCTTGTTGCTCGTGGGTAACGGCAATCAAACTTATCACCCCAACACAACGATCCTGTAAA  >  NC_000913/3005213‑3005475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A