Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 71 51.6 2219666 95.9% 2128659 118.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,607,066 A→G V212A (GTT→GCT)  rsmC ← 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,607,0660AG100.0% 73.9 / NA 21V212A (GTT→GCT) rsmC16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

CGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGC  >  NC_000913/4606932‑4607171
                                                                                                                                      |                                                                                                         
cGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGa                                                                                                       >  1:862744/1‑139 (MQ=255)
        aCGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGc                                                                                               >  1:345045/1‑139 (MQ=255)
         cGTTGCTGGCTAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGcc                                                                                              <  1:295676/139‑1 (MQ=255)
         cGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGcc                                                                                              <  1:372719/139‑1 (MQ=255)
          gTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGCGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGccc                                                                                             >  1:146086/1‑139 (MQ=255)
           ttGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCcg                                                                                            >  2:1007871/1‑139 (MQ=255)
              cTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCgcgc                                                                                         <  2:345045/139‑1 (MQ=255)
                 gCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTGCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCcac                                                                                      <  2:482432/139‑1 (MQ=255)
                    aaGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCTCGCCACAGc                                                                                   >  1:656528/1‑139 (MQ=255)
                                tCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCACCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCa                                                                       >  1:441783/1‑139 (MQ=255)
                                 cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCAc                                                                      <  2:862943/139‑1 (MQ=255)
                                                cAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTTCCTTTcctttg                                                       <  1:644926/139‑6 (MQ=255)
                                                cAAGTGTTGCGCGGC‑GGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGc                                                      <  2:292211/139‑1 (MQ=255)
                                                             gctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAg                                                  <  1:764548/131‑1 (MQ=255)
                                                             gctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAg                                                  >  2:764548/1‑131 (MQ=255)
                                                              ctggctTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCg                                         >  1:105692/1‑139 (MQ=255)
                                                                   ttCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGagc                                    <  1:1007871/139‑1 (MQ=255)
                                                                               ggCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTa                        <  2:105692/139‑1 (MQ=255)
                                                                                 cAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTAcc                      <  2:441783/139‑1 (MQ=255)
                                                                                 cAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTAcc                      <  2:59216/139‑1 (MQ=255)
                                                                                                     agaCGAATTTTCGGCGAATGGCGCGCAAAGGCAGCTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGc  >  2:549333/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                         
CGGAAAAGACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCCGACATCCAGCACTTTACCTTTCGTGTGCGGAGTTAACGTCGAGAGCAGCAACTGGCTACCGACATCCAGACCGTCGCGGC  >  NC_000913/4606932‑4607171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A