Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,135,962	T→A	D46V (GAT→GTT)	wzb ←	colanic acid production protein‑tyrosine‑phosphatase, Wzc‑P dephosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,135,962	0	T	A	100.0%	150.6 / NA	43	D46V (GAT→GTT)	wzb	colanic acid production protein‑tyrosine‑phosphatase, Wzc‑P dephosphorylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (22/21); total (22/21)

GTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGATCAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGATG  >  NC_000913/2135834‑2136087

gTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCtt                                                                                                                     >  2:107875/1‑139 (MQ=255)

 ttCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTa                                                                                                                    >  2:764741/1‑139 (MQ=255)

                      ggTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCa                                                                                               >  1:720243/1‑139 (MQ=255)

                       gTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAg                                                                                              <  1:629851/139‑1 (MQ=255)

                             aTCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACt                                                                                        <  1:548642/139‑1 (MQ=255)

                                  gTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACt                                                                                   >  1:858596/1‑139 (MQ=255)

                                   tCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACtt                                                                                  <  2:327003/139‑1 (MQ=255)

                                      tAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCa                                                                               >  2:346746/1‑139 (MQ=255)

                                       aGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAg                                                                              >  2:197193/1‑139 (MQ=255)

                                         ttGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCt                                                                            <  2:494037/139‑1 (MQ=255)

                                              acacAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCggg                                                                       <  2:840703/139‑1 (MQ=255)

                                                   gACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATa                                                                  >  2:1026374/1‑139 (MQ=255)

                                                   gACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATa                                                                  >  1:908117/1‑139 (MQ=255)

                                                    aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACttt                                                                                 <  2:463978/123‑1 (MQ=255)

                                                    aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACttt                                                                                 >  1:463978/1‑123 (MQ=255)

                                                    aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATaa                                                                 >  2:888197/1‑139 (MQ=255)

                                                                       gCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTccg                                              <  1:764741/139‑1 (MQ=255)

                                                                       gCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTccg                                              <  2:720243/139‑1 (MQ=255)

                                                                             tGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCg                                        >  2:607642/1‑139 (MQ=255)

                                                                                      agagACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGc                               <  2:132612/139‑1 (MQ=255)

                                                                                       gagaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCa                              >  2:356154/1‑139 (MQ=255)

                                                                                         gaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCggg                                      <  1:286167/129‑1 (MQ=255)

                                                                                         gaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCggg                                      >  2:286167/1‑129 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTc                                                                           <  1:694444/87‑1 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTc                                                                           >  2:694444/1‑87 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:684515/1‑139 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:2092/1‑139 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  2:9921/1‑139 (MQ=255)

                                                                                              ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACACCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:745530/1‑139 (MQ=255)

                                                                                                   gTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGac                  <  1:607642/139‑1 (MQ=255)

                                                                                                     tCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGacac                <  1:197193/139‑1 (MQ=255)

                                                                                                      cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAat                           >  2:1027996/1‑127 (MQ=255)

                                                                                                      cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAat                           <  1:1027996/127‑1 (MQ=255)

                                                                                                      cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               <  2:147132/139‑1 (MQ=255)

                                                                                                        gcggcgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGa             <  2:60377/139‑1 (MQ=255)

                                                                                                         cggcgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGAc            >  2:466145/1‑139 (MQ=255)

                                                                                                            cgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAAc         >  1:251570/1‑139 (MQ=255)

                                                                                                                gCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAg     <  1:657105/139‑1 (MQ=255)

                                                                                                                 cTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGa    <  1:888197/139‑1 (MQ=255)

                                                                                                                 cTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGa    <  2:908117/139‑1 (MQ=255)

                                                                                                                   gATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGAtg  <  1:9921/139‑1 (MQ=255)

                                                                                                                          gTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               >  1:908362/1‑119 (MQ=255)

                                                                                                                          gTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               <  2:908362/119‑1 (MQ=255)

GTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGATCAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGATG  >  NC_000913/2135834‑2136087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A