Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,895,747	C→T	E11E (GAG→GAA)	ygcQ ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,895,747	0	C	T	100.0%	120.0 / NA	36	E11E (GAG→GAA)	ygcQ	putative flavoprotein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/18); total (18/18)

CGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCT  >  NC_000913/2895615‑2895878

cGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGa                                                                                                                               >  2:90307/1‑139 (MQ=255)

  ttCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTAAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAtt                                                                                                                             >  1:358176/1‑139 (MQ=255)

           aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTcac                                                                                                                    >  1:238762/1‑139 (MQ=255)

                  caccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                             <  2:318883/139‑1 (MQ=255)

                   accaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGa                                                                                                            >  2:981531/1‑139 (MQ=255)

                       aCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATAtt                                                                                                        >  2:1028194/1‑139 (MQ=255)

                        cGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTc                                                                                                       <  2:320594/139‑1 (MQ=255)

                           aTCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTAAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATg                                                                                                    <  2:358176/139‑1 (MQ=255)

                            tCCAGCACCTGTTCCGCCACTGCAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATgg                                                                                                   <  2:658007/139‑1 (MQ=255)

                                             cACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGACGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCtttacgcca                                                                                  <  1:981531/139‑7 (MQ=255)

                                               cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTaaa                                                                                <  2:31592/139‑1 (MQ=255)

                                               cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTaaa                                                                                <  2:238762/139‑1 (MQ=255)

                                                    ggCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           >  2:239812/1‑139 (MQ=255)

                                                         aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:627633/1‑119 (MQ=255)

                                                         aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:627633/119‑1 (MQ=255)

                                                         aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                      >  2:758054/1‑139 (MQ=255)

                                                                   tGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCATAAATAGTCCTGCCATAGCTTTTg                                                            <  1:758054/139‑1 (MQ=255)

                                                                    gCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           >  1:875521/1‑123 (MQ=255)

                                                                    gCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                           <  2:875521/123‑1 (MQ=255)

                                                                         tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAt                                                                                                                              <  1:146877/67‑1 (MQ=255)

                                                                         tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGAt                                                                                                                              >  2:146877/1‑67 (MQ=255)

                                                                                      aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:669133/90‑1 (MQ=255)

                                                                                      aaCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:669133/1‑90 (MQ=255)

                                                                                       aCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGttt                                        <  1:898701/139‑1 (MQ=255)

                                                                                       aCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGttt                                        <  1:1095141/139‑1 (MQ=255)

                                                                                           cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGc                                                                                               >  2:1032282/1‑80 (MQ=255)

                                                                                           cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGc                                                                                               <  1:1032282/80‑1 (MQ=255)

                                                                                           cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGccc                                    >  2:572446/1‑139 (MQ=255)

                                                                                           cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGccc                                    >  1:1072668/1‑139 (MQ=255)

                                                                                            tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                      <  1:723305/72‑1 (MQ=255)

                                                                                            tGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                      >  2:723305/1‑72 (MQ=255)

                                                                                                      cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          <  2:1094675/74‑1 (MQ=255)

                                                                                                      cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                          >  1:1094675/1‑74 (MQ=255)

                                                                                                                   cTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcg            <  1:572446/139‑1 (MQ=255)

                                                                                                                           tGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCCCGTCGTtgctctg    >  1:726651/1‑139 (MQ=255)

                                                                                                                             cGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgctctgct  >  1:325584/1‑139 (MQ=255)

CGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCT  >  NC_000913/2895615‑2895878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A