Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
78 |
48.0 |
2243616 |
94.8% |
2126947 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,428,954 |
G→A |
intergenic (‑192/‑282) |
rrsD ← / → yrdA |
16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,428,954 | 0 | G | A | 100.0%
| 115.7
/ NA
| 34 | intergenic (‑192/‑282) | rrsD/yrdA | 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/18); total (16/18) |
TATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCAC > NC_000913/3428860‑3429081
|
tATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTtct > 1:105371/1‑139 (MQ=18)
tCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCg < 2:320478/139‑1 (MQ=37)
tCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCg < 1:953665/139‑1 (MQ=37)
cccACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTcc > 1:612398/1‑139 (MQ=255)
cacacaGATTGTCTGATAAATAGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGa > 1:673718/1‑139 (MQ=255)
acaGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCa > 1:925535/1‑139 (MQ=255)
aTTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCt > 1:1090341/1‑139 (MQ=255)
ttGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCgttgt > 2:931254/1‑126 (MQ=255)
ttGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCgttgt < 1:931254/126‑1 (MQ=255)
tGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAg > 1:1083422/1‑139 (MQ=255)
aTAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGaa < 2:94939/136‑1 (MQ=255)
aTAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGaa > 1:94939/1‑136 (MQ=255)
tGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCAc > 1:73060/1‑139 (MQ=255)
tGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCAc > 1:143332/1‑139 (MQ=255)
ttAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCg > 1:943322/1‑107 (MQ=255)
ttAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCg < 2:943322/107‑1 (MQ=255)
agCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTgtcgt < 2:143332/139‑1 (MQ=255)
cAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATc < 1:442687/113‑1 (MQ=255)
cAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATc > 2:442687/1‑113 (MQ=255)
gTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATc > 2:1085420/1‑111 (MQ=255)
gTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATc < 1:1085420/111‑1 (MQ=255)
ttGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCaa < 2:1090341/139‑1 (MQ=255)
aCGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAg < 2:73060/139‑1 (MQ=255)
aCGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAg < 1:721922/139‑1 (MQ=255)
cgcgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAgg < 2:105371/139‑1 (MQ=255)
cgcgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAgg < 1:242903/139‑1 (MQ=255)
cgcgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAgg < 2:983522/139‑1 (MQ=255)
gcgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGgc < 2:525098/139‑1 (MQ=255)
cgGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGgcg < 2:787565/139‑1 (MQ=255)
cgGCTTTCAGCTCACTGTCGCGAGGTGGCATATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGgcg < 2:188165/139‑1 (MQ=255)
gCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCa > 2:71283/1‑139 (MQ=255)
tGTCGCGAGGTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATccc > 2:380107/1‑139 (MQ=255)
gTGGCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTg < 2:612398/139‑1 (MQ=255)
gCGTATATTACACTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGcac > 2:315501/1‑139 (MQ=255)
|
TATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACTGTCGCGAGGTGGCGTATATTACGCTTTCCTCTTTCAGAGTCAACCCTGAATTTCAGGATTTTTTTCTCTTCGTTGTTCCGACCATCCTGTGAAGTGTTTCACGTTGTCGTCTCAACGGAGGCGCATTATAGGGATCCCAATTTTTTGCAC > NC_000913/3428860‑3429081
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
